Protein kinase Cα-mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it.
NetphosK3.1
PKCalpha
Twist1
embryonic development
epithelial-mesenchymal transition (EMT)
ovarian cancer
phosphorylation
post-transcriptional regulation
ubiquitylation (ubiquitination)
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
29 03 2019
29 03 2019
Historique:
received:
19
09
2018
revised:
31
01
2019
pubmed:
9
2
2019
medline:
18
10
2019
entrez:
9
2
2019
Statut:
ppublish
Résumé
Twist1 is a basic helix-loop-helix transcription factor that plays a key role in embryonic development, and its expression is down-regulated in adult cells. However, Twist1 is highly expressed during cancer development, conferring a proliferative, migratory, and invasive phenotype to malignant cells. Twist1 expression can be regulated post-translationally by phosphorylation or ubiquitination events. We report in this study a previously unknown and relevant Twist1 phosphorylation site that controls its stability. To identify candidate phosphorylation sites in Twist1, we first conducted an
Identifiants
pubmed: 30733340
pii: S0021-9258(20)35558-7
doi: 10.1074/jbc.RA118.005921
pmc: PMC6442047
doi:
Substances chimiques
Nuclear Proteins
0
TWIST1 protein, human
0
Twist-Related Protein 1
0
PRKCA protein, human
EC 2.7.11.13
Protein Kinase C-alpha
EC 2.7.11.13
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5082-5093Subventions
Organisme : NCI NIH HHS
ID : R01 CA199004
Pays : United States
Informations de copyright
© 2019 Tedja et al.
Références
Mol Cell Biol. 2015 Dec;35(24):4096-109
pubmed: 26391953
Mol Pharmacol. 2015 May;87(5):832-41
pubmed: 25724832
J Exp Clin Cancer Res. 2010 Aug 05;29:104
pubmed: 20684793
Cell. 2003 Jan 24;112(2):169-80
pubmed: 12553906
BMC Cancer. 2009 Jul 18;9:240
pubmed: 19615090
Oncogene. 2006 Jun 1;25(23):3286-95
pubmed: 16407820
Discov Med. 2013 Jan;15(80):7-15
pubmed: 23375009
Hum Mol Genet. 2000 Mar 22;9(5):813-9
pubmed: 10749989
Trends Genet. 1997 Oct;13(10):384-7
pubmed: 9351337
Cancer Res. 2003 Apr 15;63(8):1906-13
pubmed: 12702582
J Bone Miner Res. 2014 Aug;29(8):1886-99
pubmed: 24619707
Clin Cancer Res. 2014 Feb 15;20(4):951-61
pubmed: 24352647
Oncogene. 2010 Jun 17;29(24):3554-65
pubmed: 20400976
J Biol Chem. 2008 May 23;283(21):14665-73
pubmed: 18353781
Oncotarget. 2015 Aug 28;6(25):20977-92
pubmed: 26023795
Nat Genet. 2005 Apr;37(4):373-81
pubmed: 15735646
Proteomics. 2004 Jun;4(6):1633-49
pubmed: 15174133
Oncogene. 2017 Feb 23;36(8):1157-1166
pubmed: 27524420
Cancer Med. 2013 Dec;2(6):751-62
pubmed: 24403249
PLoS One. 2011;6(12):e29758
pubmed: 22242143
Cancer Cell. 2014 Feb 10;25(2):210-25
pubmed: 24525235
Am J Hum Genet. 1998 Nov;63(5):1282-93
pubmed: 9792856
Int J Dev Biol. 2002;46(4):401-13
pubmed: 12141426
Cell Growth Differ. 1999 May;10(5):343-52
pubmed: 10359015
Oncotarget. 2014 Sep 15;5(17):7260-71
pubmed: 25238494
Oncology. 2004;67(1):1-10
pubmed: 15459489
Am J Physiol Gastrointest Liver Physiol. 2008 Apr;294(4):G899-905
pubmed: 18239055
Endocr Relat Cancer. 2017 Apr;24(4):171-180
pubmed: 28223364
Dev Biol. 1997 Sep 15;189(2):205-14
pubmed: 9299114
Nat Genet. 1997 Jan;15(1):36-41
pubmed: 8988166
Oncotarget. 2017 Aug 24;8(43):75127-75140
pubmed: 29088851
Cancer Res. 2001 Jun 1;61(11):4612-9
pubmed: 11389098
Nat Methods. 2014 Aug;11(8):783-784
pubmed: 25075903
Neoplasia. 2015 Jan;17(1):16-31
pubmed: 25622896
Oncogene. 2003 May 1;22(17):2611-20
pubmed: 12730675
Oncogene. 2013 Jan 3;32(1):39-49
pubmed: 22349827
Oncogene. 2008 Aug 7;27(34):4712-23
pubmed: 18408758
Cancer Cell. 2013 Sep 9;24(3):347-64
pubmed: 24029232
BMC Bioinformatics. 2012 Jul 28;13:184
pubmed: 22839202
J Clin Invest. 1995 Apr;95(4):1906-15
pubmed: 7706498
Cancer Res. 2011 Jun 1;71(11):3980-90
pubmed: 21502402
Mol Endocrinol. 2007 Nov;21(11):2805-20
pubmed: 17684115
Cell. 2004 Jun 25;117(7):927-39
pubmed: 15210113
Cell Cycle. 2009 Jan 1;8(1):158-66
pubmed: 19158483
J Biol Chem. 2003 Sep 5;278(36):34709-16
pubmed: 12794082
Oncogene. 2014 Nov 6;33(45):5225-37
pubmed: 24336328