Pharmacogenomic-pharmacokinetic study of selective estrogen-receptor modulators with intra-patient dose escalation in breast cancer.


Journal

Breast cancer (Tokyo, Japan)
ISSN: 1880-4233
Titre abrégé: Breast Cancer
Pays: Japan
ID NLM: 100888201

Informations de publication

Date de publication:
Sep 2019
Historique:
received: 14 06 2018
accepted: 03 02 2019
pubmed: 9 2 2019
medline: 1 1 2020
entrez: 9 2 2019
Statut: ppublish

Résumé

An association between CYP2D6 polymorphisms and tamoxifen (TAM) efficacy has not been confirmed, partly due to unreliable prediction of active metabolite exposure solely by CYP2D6 activity. The efficacy of TAM dose escalation appears limited in poor TAM metabolizers. Since the chlorine atom on the side chain of toremifene (TOR) prevents 4-hydroxylation by CYP2D6, its contribution to active conversion of TOR is minor. We examined the role of TOR and its dose escalation among poor TAM metabolizers. The pharmacokinetics (PK) and pharmacogenomics (PGx) of TAM and TOR were studied. Correlation between PK and CYP2D6 inhibitor use, smoking status, and PGx were examined by regression analysis. For patients showing low endoxifen levels, an intra-patient dose escalation of TOR was conducted, and TOR was increased from 40 to 120 mg for ≥ 24 weeks with PK sampling. Total activity was calculated as the sum of the concentration of each active metabolite adjusted by their respective in vitro activities. Fifty and 11 of the 273 participating patients had endoxifen levels < 15 and < 7.5 ng/mL, respectively. The CYP2D6 genotype was the major determinant for TAM activity (p < 0.01). Smoking status (p = 0.07) and the CYP2C19 phenotype (p = 0.07), but not the CYP2D6 genotype (p = 0.61), showed marginally significant effects on TOR activity. TOR activity increased significantly with dose escalation, even among poor TAM metabolizers, and was maintained for ≥ 24 weeks. TOR might be a valid alternative to TAM in patients predicted to be poor TAM metabolizers.

Sections du résumé

BACKGROUND BACKGROUND
An association between CYP2D6 polymorphisms and tamoxifen (TAM) efficacy has not been confirmed, partly due to unreliable prediction of active metabolite exposure solely by CYP2D6 activity. The efficacy of TAM dose escalation appears limited in poor TAM metabolizers. Since the chlorine atom on the side chain of toremifene (TOR) prevents 4-hydroxylation by CYP2D6, its contribution to active conversion of TOR is minor. We examined the role of TOR and its dose escalation among poor TAM metabolizers.
METHODS METHODS
The pharmacokinetics (PK) and pharmacogenomics (PGx) of TAM and TOR were studied. Correlation between PK and CYP2D6 inhibitor use, smoking status, and PGx were examined by regression analysis. For patients showing low endoxifen levels, an intra-patient dose escalation of TOR was conducted, and TOR was increased from 40 to 120 mg for ≥ 24 weeks with PK sampling. Total activity was calculated as the sum of the concentration of each active metabolite adjusted by their respective in vitro activities.
RESULTS RESULTS
Fifty and 11 of the 273 participating patients had endoxifen levels < 15 and < 7.5 ng/mL, respectively. The CYP2D6 genotype was the major determinant for TAM activity (p < 0.01). Smoking status (p = 0.07) and the CYP2C19 phenotype (p = 0.07), but not the CYP2D6 genotype (p = 0.61), showed marginally significant effects on TOR activity. TOR activity increased significantly with dose escalation, even among poor TAM metabolizers, and was maintained for ≥ 24 weeks.
CONCLUSION CONCLUSIONS
TOR might be a valid alternative to TAM in patients predicted to be poor TAM metabolizers.

Identifiants

pubmed: 30734152
doi: 10.1007/s12282-019-00952-9
pii: 10.1007/s12282-019-00952-9
pmc: PMC6694038
doi:

Substances chimiques

Cytochrome P-450 CYP2D6 Inhibitors 0
Selective Estrogen Receptor Modulators 0
Tamoxifen 094ZI81Y45
4-hydroxy-N-desmethyltamoxifen 46AF8680RC
Toremifene 7NFE54O27T
Cytochrome P-450 CYP2C19 EC 1.14.14.1
Cytochrome P-450 CYP2D6 EC 1.14.14.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

535-543

Subventions

Organisme : Japan Breast Cancer Research Group
ID : JBCRG12
Organisme : Japan Breast Cancer Research Group
ID : JBCRG15

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Auteurs

Hiroshi Ishiguro (H)

Department of Medical Oncology, International University of Health and Welfare Hospital, 537-3 Iguchi, Nasushiobara, Tochigi, 329-2763, Japan. hishiguro@iuhw.ac.jp.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan. hishiguro@iuhw.ac.jp.

Shinji Ohno (S)

Center of Breast Oncology, The Cancer Institute Hospital of JFCR, Tokyo, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Yutaka Yamamoto (Y)

Department of Molecular-Targeting Therapy for Breast Cancer, Kumamoto University Hospital, Kumamoto, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Shintaro Takao (S)

Department of Breast Surgery, Hyogo Cancer Center, Akashi, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Nobuaki Sato (N)

Department of Breast Surgery, Niigata Cancer Center Hospital, Niigata, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Tomomi Fujisawa (T)

Department of Breast Oncology, Gunma Prefectural Cancer Center, Ohta, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Takayuki Kadoya (T)

Department of Surgical Oncology, Hiroshima University Hospital, Hiroshima, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Katsumasa Kuroi (K)

Department of Breast Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Hiroko Bando (H)

Breast and Endocrine Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Yasufumi Teramura (Y)

Department of Surgery, Hikone Municipal Hospital, Hikone, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Hiroji Iwata (H)

Department of Breast Oncology, Aichi Cancer Center Hospital, Nagoya, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

Shiro Tanaka (S)

Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Kyoto, Japan.

Masakazu Toi (M)

Department of Breast Surgery, Graduate School of Medicine Kyoto University, Kyoto, Japan.
Japan Breast Cancer Research Group (JBCRG), Tokyo, Japan.

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Classifications MeSH