Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assays for rhinovirus detection.
5' Untranslated Regions
/ genetics
DNA Primers
Fluorescence
Humans
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
/ methods
Picornaviridae Infections
/ diagnosis
RNA, Viral
/ genetics
Real-Time Polymerase Chain Reaction
Rhinovirus
/ genetics
Sensitivity and Specificity
Temperature
fluorescence
quenching primer
reverse transcription loop-mediated isothermal amplification
rhinovirus
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
04
10
2018
revised:
06
02
2019
accepted:
06
02
2019
pubmed:
9
2
2019
medline:
10
4
2020
entrez:
9
2
2019
Statut:
ppublish
Résumé
Human rhinoviruses (RVs) belong to the genus Enterovirus of the family Picornaviridae, and are classified into RV-A, -B, and -C species. Two assays were developed to detect RVs by a real-time fluorescent reverse transcription loop-mediated isothermal amplification method: one was designed based on the 5'-untranslated regions (UTRs) of RV-A and -B, and the other was designed based on the 5'-UTR of RV-C. The competence of both assays for the diagnosis of RV infection was tested using isolated viruses and compared with real-time reverse transcription polymerase chain reaction assays on clinical specimens. Neither assay demonstrated cross-reactivity with other tested enteroviruses, and they detected 19 out of 21 tested RV-As and seven out of eight tested RV-Cs. The specificity of the assays was 100% for the detection of RVs and their sensitivity for RV-A and RV-C was 86.3% and 77.3%, respectively, on clinical specimens by the combined use of both assays. Considering that both developed assays were highly specific and detected the majority of recently circulating RVs, they are helpful for the diagnosis of RV infection. Consequently, the results generated by these assays will enhance the surveillance of respiratory illness and the study of the roles of RVs associated with clinical features and disease severity.
Identifiants
pubmed: 30735248
doi: 10.1002/jmv.25427
pmc: PMC7166982
doi:
Substances chimiques
5' Untranslated Regions
0
DNA Primers
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1232-1238Informations de copyright
© 2019 Wiley Periodicals, Inc.
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