The KSHV portal protein ORF43 is essential for the production of infectious viral particles.
Kaposi’s sarcoma-associated herpesvirus, KSHV
ORF43
Portal protein
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
06
09
2018
revised:
13
01
2019
accepted:
21
01
2019
pubmed:
9
2
2019
medline:
16
7
2019
entrez:
9
2
2019
Statut:
ppublish
Résumé
Herpesvirus capsid assembly involves cleavage and packaging of the viral genome. The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 43 (orf43) encodes a putative portal protein. The portal complex functions as a gate through which DNA is packaged into the preformed procapsids, and is injected into the cell nucleus upon infection. The amino acid sequence of the portal proteins is conserved among herpesviruses. Here, we generated an antiserum to ORF43 and determined late expression kinetics of ORF43 along with its nuclear localization. We generated a recombinant KSHV mutant, which fails to express ORF43 (BAC16-ORF43-null). Assembled capsids were observed upon lytic induction of this virus; however, the released virions lacked viral DNA and thus could not establish infection. Ectopic expression of ORF43 rescued the ability to produce infectious particles. ORF43 antiserum and the recombinant ORF43-null virus can provide an experimental system for further studies of the portal functions and its interactions.
Identifiants
pubmed: 30735904
pii: S0042-6822(19)30024-8
doi: 10.1016/j.virol.2019.01.028
pii:
doi:
Substances chimiques
Capsid Proteins
0
DNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
205-215Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.