Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids.

Bacteriophages / genetics Capsid / ultrastructure Cryoelectron Microscopy DNA Packaging / genetics DNA Viruses / genetics DNA, Viral / genetics Virion / genetics Virus Assembly / genetics
DNA packaging capsid cryo-EM portal protein virus assembly

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
26 02 2019
Historique:
pubmed: 10 2 2019
medline: 3 5 2019
entrez: 10 2 2019
Statut: ppublish

Résumé

Double-stranded DNA viruses, including bacteriophages and herpesviruses, package their genomes into preformed capsids, using ATP-driven motors. Seeking to advance structural and mechanistic understanding, we established in vitro packaging for a thermostable bacteriophage, P23-45 of

Identifiants

DOI: 10.1073/pnas.1813204116 PMID: 30737287 PMC: PMC6397560
pubmed: 30737287
pii: 1813204116
doi: 10.1073/pnas.1813204116
pmc: PMC6397560
doi:

Substances chimiques

DNA, Viral 0

Banques de données

PDB
['6I9E', '6IBC', '6IBG']

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3556-3561

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206377/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206377
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM104071
Pays : United States

Informations de copyright

Copyright © 2019 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

Références

J Mol Biol. 2000 Jan 14;295(2):155-61
pubmed: 10623515
J Mol Biol. 2000 Feb 11;296(1):103-15
pubmed: 10656820
Nat Struct Biol. 2000 Mar;7(3):230-7
pubmed: 10700283
Science. 2000 Sep 22;289(5487):2129-33
pubmed: 11000116
J Mol Biol. 1975 Oct 15;98(1):93-106
pubmed: 1195386
EMBO J. 2003 Mar 17;22(6):1255-62
pubmed: 12628918
Cold Spring Harb Symp Quant Biol. 1962;27:1-24
pubmed: 14019094
Cell. 2005 Sep 9;122(5):683-92
pubmed: 16143101
J Mol Biol. 2006 Nov 3;363(4):786-99
pubmed: 16987527
EMBO J. 2007 Apr 4;26(7):1984-94
pubmed: 17363899
J Mol Biol. 2007 Nov 9;373(5):1113-22
pubmed: 17919653
Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16868-73
pubmed: 17942694
J Mol Biol. 2008 Apr 25;378(2):468-80
pubmed: 18355836
Structure. 2008 Sep 10;16(9):1399-406
pubmed: 18786402
Cell. 2008 Dec 26;135(7):1251-62
pubmed: 19109896
Nat Struct Mol Biol. 2008 Sep;15(9):939-47
pubmed: 19172747
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9157-62
pubmed: 19470490
J Mol Biol. 2010 Feb 19;396(2):384-95
pubmed: 19944702
PLoS One. 2010 Mar 04;5(3):e9423
pubmed: 20209096
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16078-83
pubmed: 20805464
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1355-60
pubmed: 21220301
Nat Struct Mol Biol. 2011 May;18(5):597-603
pubmed: 21499245
Nat Rev Microbiol. 2011 Aug 12;9(9):647-57
pubmed: 21836625
Structure. 2012 Mar 7;20(3):498-503
pubmed: 22405008
Curr Opin Virol. 2011 Aug;1(2):134-41
pubmed: 22440623
Cell. 2012 Nov 21;151(5):1017-28
pubmed: 23178121
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):876-9
pubmed: 23908032
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1239-41
pubmed: 24192358
EMBO J. 2014 Sep 1;33(17):1896-911
pubmed: 25024436
Nat Commun. 2015 Jul 06;6:7548
pubmed: 26144253
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3792-9
pubmed: 26150523
Cell Rep. 2016 Mar 1;14(8):2017-2029
pubmed: 26904950
Nucleic Acids Res. 2017 Apr 7;45(6):3580-3590
pubmed: 28100693
Nat Commun. 2017 Jan 30;8:14310
pubmed: 28134243
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4779-83
pubmed: 283391
MBio. 2017 Oct 17;8(5):
pubmed: 29042498
Structure. 2018 Feb 6;26(2):238-248.e3
pubmed: 29290487
Science. 2018 Apr 6;360(6384):
pubmed: 29622627
Structure. 2018 Jul 3;26(7):936-947.e3
pubmed: 29779790
Virology. 1979 Jun;95(2):550-64
pubmed: 380139
J Mol Biol. 1985 Oct 5;185(3):565-78
pubmed: 4057255
Proc Natl Acad Sci U S A. 1973 Jan;70(1):260-4
pubmed: 4509659
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3545-9
pubmed: 4530321
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372-6
pubmed: 4601587
Virology. 1978 Jun 15;87(2):394-400
pubmed: 664260
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5861-5
pubmed: 6795639
Virology. 1983 Jan 15;124(1):109-20
pubmed: 6823742
Virology. 1981 Aug;113(1):336-44
pubmed: 7269246
J Mol Biol. 1976 May 15;103(2):299-318
pubmed: 781268

Auteurs

Oliver W Bayfield (OW)

York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.
Laboratory of Structural Biology Research, National Institute of Arthritis Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
ORCID: 0000-0003-1421-7780

Evgeny Klimuk (E)

Center for Life Sciences, Skolkovo Institute of Science and Technology, 143025 Skolkovo, Russia.
Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Moscow, Russia.
ORCID: 0000-0001-5314-038X

Dennis C Winkler (DC)

Laboratory of Structural Biology Research, National Institute of Arthritis Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
ORCID: 0000-0003-1445-6554

Emma L Hesketh (EL)

Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
ORCID: 0000-0002-4522-0061

Maria Chechik (M)

York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.
ORCID: 0000-0001-8413-4230

Naiqian Cheng (N)

Laboratory of Structural Biology Research, National Institute of Arthritis Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.

Eric C Dykeman (EC)

Department of Mathematics, University of York, York YO10 5DD, United Kingdom.

Leonid Minakhin (L)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
ORCID: 0000-0003-0301-7054

Neil A Ranson (NA)

Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
ORCID: 0000-0002-3640-5275

Konstantin Severinov (K)

Center for Life Sciences, Skolkovo Institute of Science and Technology, 143025 Skolkovo, Russia.
Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Moscow, Russia.
Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
ORCID: 0000-0001-9706-450X

Alasdair C Steven (AC)

Laboratory of Structural Biology Research, National Institute of Arthritis Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
ORCID: 0000-0003-4104-6497

Alfred A Antson (AA)

York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom; fred.antson@york.ac.uk.
ORCID: 0000-0002-4533-3816

Articles similaires

Atomic view of photosynthetic metabolite permeability pathways and confinement in synthetic carboxysome shells.

Daipayan Sarkar, Christopher Maffeo, Markus Sutter et al.
1.00
Photosynthesis Ribulose-Bisphosphate Carboxylase Carbon Dioxide Molecular Dynamics Simulation Cyanobacteria

Complete genome sequences of two novel Ralstonia jumbo phages isolated from leaf litter compost.

Ryota Sasaki, Shuhei Miyashita, Hideki Takahashi
1.00
Genome, Viral Ralstonia Composting Solanum lycopersicum Bacteriophages

REliable PIcking by Consensus (REPIC): a consensus methodology for harnessing multiple cryo-EM particle pickers.

Christopher J F Cameron, Sebastian J H Seager, Fred J Sigworth et al.
1.00
Cryoelectron Microscopy Algorithms Image Processing, Computer-Assisted Consensus Software

Prediction of strain level phage-host interactions across the Escherichia genus using only genomic information.

Baptiste Gaborieau, Hugo Vaysset, Florian Tesson et al.
1.00
Host Specificity Bacteriophages Genomics Algorithms Escherichia coli

Classifications MeSH

questionsmedicales.fr › Virus › Bactériophages › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Structures virales › Virion › Nucléocapside › Capside › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Phénomènes génétiques › Empaquetage de l'ADN › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Virus › Virus à ADN › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Structures virales › Virion › Cryo-EM structure and in vitro DNA packaging...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Assemblage viral › Cryo-EM structure and in vitro DNA packaging...