Conformations and cryo-force spectroscopy of spray-deposited single-strand DNA on gold.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 02 2019
08 02 2019
Historique:
received:
18
04
2018
accepted:
16
01
2019
entrez:
10
2
2019
pubmed:
10
2
2019
medline:
9
4
2019
Statut:
epublish
Résumé
Cryo-electron microscopy can determine the structure of biological matter in vitrified liquids. However, structure alone is insufficient to understand the function of native and engineered biomolecules. So far, their mechanical properties have mainly been probed at room temperature using tens of pico-newton forces with a resolution limited by thermal fluctuations. Here we combine force spectroscopy and computer simulations in cryogenic conditions to quantify adhesion and intra-molecular properties of spray-deposited single-strand DNA oligomers on Au(111). Sub-nanometer resolution images reveal folding conformations confirmed by simulations. Lifting shows a decay of the measured stiffness with sharp dips every 0.2-0.3 nm associated with the sequential peeling and detachment of single nucleotides. A stiffness of 30-35 N m
Identifiants
pubmed: 30737410
doi: 10.1038/s41467-019-08531-4
pii: 10.1038/s41467-019-08531-4
pmc: PMC6368621
doi:
Substances chimiques
DNA, Single-Stranded
0
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
685Références
Nanoscale. 2018 Jan 18;10(3):1337-1344
pubmed: 29296988
Mass Spectrom Rev. 2009 Nov-Dec;28(6):898-917
pubmed: 19551695
J Phys Chem B. 2009 Oct 8;113(40):13279-90
pubmed: 19757835
Science. 1996 Feb 9;271(5250):795-9
pubmed: 8628994
Annu Rev Biophys Biomol Struct. 1996;25:395-429
pubmed: 8800476
Langmuir. 2006 Dec 19;22(26):11180-6
pubmed: 17154600
Langmuir. 2018 Dec 11;34(49):14749-14756
pubmed: 29723478
Science. 1989 Oct 6;246(4926):64-71
pubmed: 2675315
J Chem Theory Comput. 2012 May 8;8(5):1542-1555
pubmed: 22582031
Science. 2006 Dec 8;314(5805):1585-8
pubmed: 17158324
Nature. 1953 Apr 25;171(4356):737-8
pubmed: 13054692
Science. 2016 Feb 26;351(6276):957-61
pubmed: 26917767
Biophys J. 1995 May;68(5):1672-7
pubmed: 7612809
Nature. 2003 Jan 23;421(6921):423-7
pubmed: 12540915
Nanoscale. 2016 Jul 7;8(27):13463-75
pubmed: 27352029
Science. 2009 Aug 28;325(5944):1110-4
pubmed: 19713523
Biophys J. 2007 Jun 1;92(11):3817-29
pubmed: 17351000
Langmuir. 2007 Jun 5;23(12):6660-6
pubmed: 17489614
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):3968-72
pubmed: 24591611
Phys Rev Lett. 2012 Aug 17;109(7):076102
pubmed: 23006386
J Chem Theory Comput. 2013 Oct 8;9(10):4552-61
pubmed: 26589170
J Chem Theory Comput. 2011 Sep 13;7(9):2886-2902
pubmed: 21921995
Nat Nanotechnol. 2011 Aug 21;6(9):553-7
pubmed: 21857685
Beilstein J Nanotechnol. 2015 Sep 18;6:1927-34
pubmed: 26665062
J Chem Theory Comput. 2013 Sep 10;9(9):3878-88
pubmed: 26592383
Nat Methods. 2008 Jun;5(6):491-505
pubmed: 18511917
Science. 1996 Mar 29;271(5257):1835-7
pubmed: 8596951
Chem Soc Rev. 2016 Jan 21;45(2):412-48
pubmed: 26750724
J Phys Chem B. 2018 Jan 18;122(2):840-846
pubmed: 28945092
Science. 1994 Nov 4;266(5186):771-3
pubmed: 7973628
J Am Chem Soc. 2014 Sep 17;136(37):12947-57
pubmed: 25162693
J Chem Theory Comput. 2013 Jun 11;9(6):2733-2748
pubmed: 23914143
Prog Biophys Mol Biol. 2000;74(1-2):115-40
pubmed: 11106809
Science. 2009 Feb 27;323(5918):1193-7
pubmed: 19251624
Science. 1991 Feb 8;251(4994):640-2
pubmed: 1992517
Nat Nanotechnol. 2009 Aug;4(8):518-22
pubmed: 19662015
Nano Lett. 2008 Dec;8(12):4365-72
pubmed: 19368004
Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7049-7054
pubmed: 28634300
Science. 2011 Apr 15;332(6027):342-6
pubmed: 21493857
Phys Rev Lett. 2005 Feb 4;94(4):048301
pubmed: 15783606
ACS Nano. 2016 Jan 26;10(1):713-22
pubmed: 26571003
Biophys J. 1996 Oct;71(4):2168-76
pubmed: 8889193
Nanoscale. 2016 Jun 9;8(23):11818-26
pubmed: 26876486
Small. 2006 Apr;2(4):540-7
pubmed: 17193083
PLoS Biol. 2004 Dec;2(12):e424
pubmed: 15583715