A novel method using nuclear magnetic resonance for plasma protein binding assessment in drug discovery programs.


Journal

Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336

Informations de publication

Date de publication:
15 Apr 2019
Historique:
received: 30 10 2018
revised: 28 01 2019
accepted: 29 01 2019
pubmed: 10 2 2019
medline: 10 7 2019
entrez: 10 2 2019
Statut: ppublish

Résumé

A new methodology based on Nuclear Magnetic Resonance (NMR) was developed to determine plasma protein binding (PPB) of drug candidates in drug discovery programs. A strong correlation was found between the attenuation of NMR signals of diverse drugs in the presence of different plasma concentrations and their fraction bound (f

Identifiants

pubmed: 30738240
pii: S0731-7085(18)32370-7
doi: 10.1016/j.jpba.2019.01.049
pii:
doi:

Substances chimiques

Blood Proteins 0
Pharmaceutical Preparations 0
Recombinant Proteins 0
Small Molecule Libraries 0
Serum Albumin, Human ZIF514RVZR

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

21-29

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Mariana Gallo (M)

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy.

Sara Matteucci (S)

Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy.

Nadine Alaimo (N)

Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy.

Erica Pitti (E)

Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy.

Maria V Orsale (MV)

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy.

Vincenzo Summa (V)

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy.

Daniel O Cicero (DO)

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy; Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy. Electronic address: cicero@scienze.uniroma2.it.

Edith Monteagudo (E)

IRBM Science Park S.p.A., Via Pontina km 30.600, 00071, Pomezia, Rome, Italy. Electronic address: e.monteagudo@irbm.it.

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Classifications MeSH