Highly conserved epitopes of DENV structural and non-structural proteins: Candidates for universal vaccine targets.
Antibodies, Neutralizing
/ genetics
Antibodies, Viral
/ genetics
Dengue
/ immunology
Dengue Virus
/ genetics
Epitopes
/ genetics
Humans
Membrane Proteins
/ genetics
RNA Helicases
/ genetics
Serine Endopeptidases
/ genetics
Serogroup
Vaccines
/ genetics
Viral Envelope Proteins
/ genetics
Viral Nonstructural Proteins
/ genetics
Comparative genomics
DENV
Epitope
Serotype
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
05 May 2019
05 May 2019
Historique:
received:
26
10
2018
revised:
22
01
2019
accepted:
01
02
2019
pubmed:
11
2
2019
medline:
23
3
2019
entrez:
11
2
2019
Statut:
ppublish
Résumé
Dengue is a severe emerging arthropod borne viral disease occurring globally. Around two fifths of the world's population, or up to 3.9 billion people, are at a risk of dengue infection. Infection induces a life-long protective immunity to the homologous serotype but confers only partial and transient protection against subsequent infection caused by other serotypes. Thus, there is a need for a vaccine which is capable of providing a life- long protection against all the serotypes of dengue virus. In our study, comparative genomics of Dengue virus (DENV) was conducted to explore potential candidates for novel vaccine targets. From our analysis we successfully found 100% conserved epitopes in Envelope protein (RCPTQGE); NS3 (SAAQRRGR, PGTSGSPI); NS4A (QRTPQDNQL); NS4B (LQAKATREAQKRA) and NS5 proteins (QRGSGQV) in all DENV serotypes. Some serotype specific conserved motifs were also found in NS1, NS5, Capsid, PrM and Envelope proteins. Using comparative genomics and immunoinformatics approach, we could find conserved epitopes which can be explored as peptide vaccine candidates to combat dengue worldwide. Serotype specific epitopes can also be exploited for rapid diagnostics. All ten proteins are explored to find the conserved epitopes in DENV serotypes, thus making it the most extensively studied viral genome so far.
Identifiants
pubmed: 30738967
pii: S0378-1119(19)30098-8
doi: 10.1016/j.gene.2019.02.001
pmc: PMC7125761
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Epitopes
0
Membrane Proteins
0
NS3 protein, flavivirus
0
NS4B protein, Dengue virus
0
NS5 protein, dengue virus
0
Vaccines
0
Viral Envelope Proteins
0
Viral Nonstructural Proteins
0
Serine Endopeptidases
EC 3.4.21.-
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-25Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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