Site-specific variations in cutaneous autofluorescence revealed by excitation-emission matrix spectroscopy.
bilayer fluorescence
cutaneous autofluorescence
excitation-emission matrix
photoaging
superficial fluorescence
Journal
Photodermatology, photoimmunology & photomedicine
ISSN: 1600-0781
Titre abrégé: Photodermatol Photoimmunol Photomed
Pays: England
ID NLM: 9013641
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
10
09
2018
accepted:
03
02
2019
pubmed:
11
2
2019
medline:
4
6
2020
entrez:
11
2
2019
Statut:
ppublish
Résumé
Although cutaneous autofluorescence has been utilized for evaluation of skin conditions, there is a paucity of data on normal human skin autofluorescence and its dependence on anatomical site. The objective of this study is to use excitation-emission matrix spectroscopy to quantify and characterize skin autofluorescence at different body sites. Ten anatomical sites from 30 healthy volunteers were measured with a double-grating excitation-emission matrix spectrofluorometer. For the 10 body sites evaluated, there were four overall patterns of autofluorescence: skin from the head and neck exhibits high superficial and low bilayer fluorescence; the dorsal forearm and dorsal hand have both low superficial and bilayer fluorescence; the upper inner arm and back have high superficial and intermediate bilayer fluorescence; while the palm and thumbnail have both high superficial and bilayer fluorescence. The corresponding fluorescence excitation-emission peaks for these patterns were as follows: head and neck, 3 peaks at 290-300/330-350, 360-380/460-485, and 380-420/610-630 nm; dorsal forearm and dorsal hand, 2 peaks around 295-300/345-360 and 385-395/460-485 nm; upper inner arm and back, 3 peaks around 295-300/335-355, 335-340/390-410, and 375-390/455-480 nm; palm and thumbnail, 3 peaks around 285-300/345-355, 335-345/390-410, and 365-390/450-480 nm. Cutaneous fluorescence varies in distinct patterns according to anatomical site, due to the component fluorophores present, skin thickness, and the degree of melanization and long term sun exposure. These EEM patterns for normal skin should be accounted for when interpreting fluorescence signals from disease states and can also be used to guide the selection of optimal wavebands when applying this optical modality.
Sections du résumé
BACKGROUND AND PURPOSE
OBJECTIVE
Although cutaneous autofluorescence has been utilized for evaluation of skin conditions, there is a paucity of data on normal human skin autofluorescence and its dependence on anatomical site. The objective of this study is to use excitation-emission matrix spectroscopy to quantify and characterize skin autofluorescence at different body sites.
METHODS
METHODS
Ten anatomical sites from 30 healthy volunteers were measured with a double-grating excitation-emission matrix spectrofluorometer.
RESULTS
RESULTS
For the 10 body sites evaluated, there were four overall patterns of autofluorescence: skin from the head and neck exhibits high superficial and low bilayer fluorescence; the dorsal forearm and dorsal hand have both low superficial and bilayer fluorescence; the upper inner arm and back have high superficial and intermediate bilayer fluorescence; while the palm and thumbnail have both high superficial and bilayer fluorescence. The corresponding fluorescence excitation-emission peaks for these patterns were as follows: head and neck, 3 peaks at 290-300/330-350, 360-380/460-485, and 380-420/610-630 nm; dorsal forearm and dorsal hand, 2 peaks around 295-300/345-360 and 385-395/460-485 nm; upper inner arm and back, 3 peaks around 295-300/335-355, 335-340/390-410, and 375-390/455-480 nm; palm and thumbnail, 3 peaks around 285-300/345-355, 335-345/390-410, and 365-390/450-480 nm.
CONCLUSION
CONCLUSIONS
Cutaneous fluorescence varies in distinct patterns according to anatomical site, due to the component fluorophores present, skin thickness, and the degree of melanization and long term sun exposure. These EEM patterns for normal skin should be accounted for when interpreting fluorescence signals from disease states and can also be used to guide the selection of optimal wavebands when applying this optical modality.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
400-407Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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