Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time-resolved X-ray scattering.
Bacterial Proteins
/ chemistry
Deinococcus
/ chemistry
Kinetics
Light
Models, Molecular
Photoreceptors, Microbial
Phytochrome
/ chemistry
Protein Conformation
/ radiation effects
Protein Kinases
/ chemistry
Scattering, Radiation
Signal Transduction
/ radiation effects
Synechocystis
/ chemistry
X-Ray Absorption Spectroscopy
X-Rays
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
14
06
2018
accepted:
20
11
2018
entrez:
12
2
2019
pubmed:
12
2
2019
medline:
12
2
2019
Statut:
epublish
Résumé
Phytochromes are photoreceptor proteins that transmit a light signal from a photosensory region to an output domain. Photoconversion involves protein conformational changes whose nature is not fully understood. Here, we use time-resolved X-ray scattering and optical spectroscopy to study the kinetics of structural changes in a full-length cyanobacterial phytochrome and in a truncated form with no output domain. X-ray and spectroscopic signals on the µs/ms timescale are largely independent of the presence of the output domain. On longer time-scales, large differences between the full-length and truncated proteins indicate the timeframe during which the structural transition is transmitted from the photosensory region to the output domain and represent a large quaternary motion. The suggested independence of the photosensory-region dynamics on the µs/ms timescale defines a time window in which the photoreaction can be characterized (e.g. for optogenetic design) independently of the nature of the engineered output domain.
Identifiants
pubmed: 30740537
doi: 10.1038/s42003-018-0242-0
pii: 242
pmc: PMC6318211
doi:
Substances chimiques
Bacterial Proteins
0
Photoreceptors, Microbial
0
Phytochrome
11121-56-5
Protein Kinases
EC 2.7.-
Cph1 phytochrome protein, bacteria
EC 2.7.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
1Déclaration de conflit d'intérêts
The authors declare no competing interests.
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