Development and characterization of novel 2'-F-RNA aptamers specific to human total and glycated hemoglobins.
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
11
12
2018
revised:
05
02
2019
accepted:
06
02
2019
pubmed:
12
2
2019
medline:
18
12
2019
entrez:
12
2
2019
Statut:
ppublish
Résumé
Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2'-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2'-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.
Identifiants
pubmed: 30742800
pii: S0003-2697(18)31232-6
doi: 10.1016/j.ab.2019.02.004
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Glycated Hemoglobin A
0
Hemoglobins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-50Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.