Sestrin-like protein from Dictyostelium discoideum is involved in autophagy under starvation stress.

Autophagy Dictyostelium / metabolism Gene Knockout Techniques Heat-Shock Proteins / genetics Mutation Reactive Oxygen Species / metabolism Sequence Alignment Starvation Stress, Psychological TOR Serine-Threonine Kinases
AMPK Autophagy Dictyostelium ROS Sestrin Stress

Journal

Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794

Informations de publication

Date de publication:
Mar 2019
Historique:
received: 23 02 2018
revised: 12 12 2018
accepted: 28 12 2018
entrez: 13 2 2019
pubmed: 13 2 2019
medline: 2 3 2019
Statut: ppublish

Résumé

Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukaryote where starvation stress initiates multicellular development. The single DdSesn-like gene was expressed and its endogenous functions were characterized. Both, the knockout and constitutively expressing strains were made and their involvement in starvation-induced autophagy was analyzed. Autophagic fluxes and ROS levels were also monitored. Additionally, overexpression of DdSesn decreased cell growth and showed a longer lag phase. Upon starvation both DdSesn and ROS levels increased. Sesn

Identifiants

DOI: 10.1016/j.micres.2018.12.006 PMID: 30744820
pubmed: 30744820
pii: S0944-5013(18)30230-1
doi: 10.1016/j.micres.2018.12.006
pii:
doi:

Substances chimiques

Heat-Shock Proteins 0
Reactive Oxygen Species 0
TOR Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

61-71

Informations de copyright

Copyright © 2018 Elsevier GmbH. All rights reserved.

Auteurs

S Rafia (S)

School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: rafia267@gmail.com.

S Saran (S)

School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: ssaran@mail.jnu.ac.in.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Autophagie › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Eucaryotes › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-out de gènes › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Protéines du choc thermique › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Troubles nutritionnels › Malnutrition › Inanition › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Phénomènes psychologiques › Stress psychologique › Sestrin-like protein from Dictyostelium...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Sérine-thréonine kinases TOR › Sestrin-like protein from Dictyostelium...