HPV as a marker for molecular characterization in head and neck oncology: Looking for a standardization of clinical use and of detection method(s) in clinical practice.
Biomarkers, Tumor
/ analysis
Carcinoma, Squamous Cell
/ diagnosis
Female
Genes, p16
Head and Neck Neoplasms
/ genetics
Humans
In Situ Hybridization
Male
Neoplasm Invasiveness
/ pathology
Neoplasm Staging
Oropharyngeal Neoplasms
/ diagnosis
Papillomaviridae
/ genetics
Papillomavirus Infections
/ complications
RNA, Messenger
/ genetics
RNA, Viral
/ genetics
Real-Time Polymerase Chain Reaction
/ methods
Risk Assessment
FFPE
HPV
molecular characterization
oropharynx
treatment deintensification
Journal
Head & neck
ISSN: 1097-0347
Titre abrégé: Head Neck
Pays: United States
ID NLM: 8902541
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
01
05
2018
accepted:
07
12
2018
pubmed:
13
2
2019
medline:
10
10
2020
entrez:
13
2
2019
Statut:
ppublish
Résumé
A consensus about the most appropriate diagnostic method(s) for head and neck human papillomavirus (HPV)-induced carcinogenesis is still lacking because most of the commercially available assays have been designed for the cervix. This article summarizes current data and trends concerning HPV diagnostic strategies in oropharyngeal squamous cell carcinoma (OPSCC). Six main approaches are described. The diagnostic gold standard for HPV-related OPSCC, focusing on E6/E7 mRNA detection, requires fresh samples. Because most frequently available samples are formalin-fixed paraffin-embedded (FFPE), the pros and cons of the different approaches were analyzed. In the FFPE samples, the immunohistochemistry of p16, which is considered appropriate to assess HPV-driven carcinogenesis in OPSCC according to the 8th American Joint Committee on Cancer TNM classification, may not be specific enough to become the diagnostic standard in the perspective of treatment deintensification. p16 may play a safer role in combination with another highly sensible assay. Other promising approaches are based on DNA detection through real-time polymerase chain reaction and RNAscope.
Sections du résumé
BACKGROUND
A consensus about the most appropriate diagnostic method(s) for head and neck human papillomavirus (HPV)-induced carcinogenesis is still lacking because most of the commercially available assays have been designed for the cervix.
METHODS
This article summarizes current data and trends concerning HPV diagnostic strategies in oropharyngeal squamous cell carcinoma (OPSCC). Six main approaches are described.
RESULTS
The diagnostic gold standard for HPV-related OPSCC, focusing on E6/E7 mRNA detection, requires fresh samples. Because most frequently available samples are formalin-fixed paraffin-embedded (FFPE), the pros and cons of the different approaches were analyzed.
CONCLUSIONS
In the FFPE samples, the immunohistochemistry of p16, which is considered appropriate to assess HPV-driven carcinogenesis in OPSCC according to the 8th American Joint Committee on Cancer TNM classification, may not be specific enough to become the diagnostic standard in the perspective of treatment deintensification. p16 may play a safer role in combination with another highly sensible assay. Other promising approaches are based on DNA detection through real-time polymerase chain reaction and RNAscope.
Substances chimiques
Biomarkers, Tumor
0
RNA, Messenger
0
RNA, Viral
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1104-1111Subventions
Organisme : World Health Organization
ID : 001
Pays : International
Informations de copyright
© 2019 Wiley Periodicals, Inc.