Formoterol counteracts the inhibitory effect of cigarette smoke on glucocorticoid-induced leucine zipper (GILZ) transactivation in human bronchial smooth muscle cells.
Active Transport, Cell Nucleus
/ drug effects
Beclomethasone
/ analogs & derivatives
Bronchi
/ cytology
Cell Nucleus
/ drug effects
Cigarette Smoking
/ adverse effects
Formoterol Fumarate
/ pharmacology
Humans
Myocytes, Smooth Muscle
/ drug effects
Phosphorylation
/ drug effects
Receptors, Glucocorticoid
/ metabolism
Transcription Factors
/ genetics
Transcriptional Activation
/ drug effects
17-beclomethasone monopropionate (BMP)
Cigarette smoke
Fluticasone propionate (FP)
GC-induced leucine zipper (GILZ)
Glucocorticoid (GC)
Glucocorticoids
Human bronchial smooth muscle cells (HBSMC)
Long-acting β2-adrenoreceptor agonist (LABA)
Journal
European journal of pharmacology
ISSN: 1879-0712
Titre abrégé: Eur J Pharmacol
Pays: Netherlands
ID NLM: 1254354
Informations de publication
Date de publication:
05 May 2019
05 May 2019
Historique:
received:
15
06
2018
revised:
06
02
2019
accepted:
08
02
2019
pubmed:
13
2
2019
medline:
7
8
2019
entrez:
13
2
2019
Statut:
ppublish
Résumé
Cigarette smokers with asthma and chronic obstructive pulmonary disease (COPD) are less responsive to glucocorticoids (GCs). The anti-inflammatory action of GCs depends also on their ability to transactivate genes such as GC-induced leucine zipper (GILZ). We investigated the effects of aqueous cigarette smoke extract (CSE) on GILZ transactivation evoked by 17-beclomethasone monopropionate (BMP) or fluticasone propionate (FP) in the presence or absence of the long acting β2-adrenoceptor agonist (LABA) bronchodilator formoterol or salmeterol in human primary cultures of human bronchial smooth muscle cells (HBSMC). We monitored GC receptor Ser211 phosphorylation by western blot analysis and GC receptor nuclear translocation by immunostaining followed high-content imaging analysis. BMP, as well as FP, induced GILZ expression in a concentration-dependent manner (EC
Identifiants
pubmed: 30753866
pii: S0014-2999(19)30101-3
doi: 10.1016/j.ejphar.2019.02.010
pii:
doi:
Substances chimiques
Receptors, Glucocorticoid
0
TSC22D3 protein, human
0
Transcription Factors
0
beclomethasone 17-monopropionate
5BGA9FD55H
Beclomethasone
KGZ1SLC28Z
Formoterol Fumarate
W34SHF8J2K
Types de publication
Journal Article
Langues
eng
Pagination
8-14Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.