CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
12 02 2019
Historique:
received: 12 10 2018
revised: 17 12 2018
accepted: 17 01 2019
entrez: 14 2 2019
pubmed: 14 2 2019
medline: 22 4 2020
Statut: ppublish

Résumé

Infection by rapidly growing Mycobacterium abscessus is increasingly prevalent in cystic fibrosis (CF), a genetic disease caused by a defective CF transmembrane conductance regulator (CFTR). However, the potential link between a dysfunctional CFTR and vulnerability to M. abscessus infection remains unknown. Herein, we exploit a CFTR-depleted zebrafish model, recapitulating CF immuno-pathogenesis, to study the contribution of CFTR in innate immunity against M. abscessus infection. Loss of CFTR increases susceptibility to infection through impaired NADPH oxidase-dependent restriction of intracellular growth and reduced neutrophil chemotaxis, which together compromise granuloma formation and integrity. As a consequence, extracellular multiplication of M. abscessus expands rapidly, inducing abscess formation and causing lethal infections. Because these phenotypes are not observed with other mycobacteria, our findings highlight the crucial and specific role of CFTR in the immune control of M. abscessus by mounting effective oxidative responses.

Identifiants

pubmed: 30759393
pii: S2211-1247(19)30100-7
doi: 10.1016/j.celrep.2019.01.071
pii:
doi:

Substances chimiques

CFTR protein, zebrafish 0
Reactive Oxygen Species 0
Zebrafish Proteins 0
Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1828-1840.e4

Subventions

Organisme : Medical Research Council
ID : G0700091
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M004864/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N02995X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L000830/1
Pays : United Kingdom

Informations de copyright

Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

Auteurs

Audrey Bernut (A)

CNRS, UMR9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France; Bateson Centre, University of Sheffield, Sheffield, UK; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, UK. Electronic address: a.bernut@sheffield.ac.uk.

Christian Dupont (C)

CNRS, UMR9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France.

Nikolay V Ogryzko (NV)

Bateson Centre, University of Sheffield, Sheffield, UK; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, UK.

Aymeric Neyret (A)

CNRS, UMR9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France.

Jean-Louis Herrmann (JL)

2I, INSERM, UVSQ, Université Paris-Saclay, Versailles, France.

R Andres Floto (RA)

Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge, UK.

Stephen A Renshaw (SA)

Bateson Centre, University of Sheffield, Sheffield, UK; Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, UK.

Laurent Kremer (L)

CNRS, UMR9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France; INSERM, IRIM, Montpellier, France. Electronic address: laurent.kremer@irim.cnrs.fr.

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Classifications MeSH