Early detection of pre-malignant lesions in a KRAS
Alleles
Animals
Cell-Free Nucleic Acids
/ blood
Disease Models, Animal
Early Detection of Cancer
Female
Integrases
/ metabolism
Lung Neoplasms
/ blood
Male
Mice, Inbred C57BL
Mutation
/ genetics
Precancerous Conditions
/ blood
Proto-Oncogene Proteins p21(ras)
/ blood
Recombination, Genetic
/ genetics
Reproducibility of Results
Tumor Burden
X-Ray Microtomography
Circulating free DNA
Early detection
KRASG12D
Lung adenocarcinoma
Mouse model
cfDNA
Journal
Disease models & mechanisms
ISSN: 1754-8411
Titre abrégé: Dis Model Mech
Pays: England
ID NLM: 101483332
Informations de publication
Date de publication:
12 02 2019
12 02 2019
Historique:
received:
17
08
2018
accepted:
21
12
2018
entrez:
15
2
2019
pubmed:
15
2
2019
medline:
14
8
2019
Statut:
epublish
Résumé
Lung cancer is the leading cause of cancer-related death. Two-thirds of cases are diagnosed at an advanced stage that is refractory to curative treatment. Therefore, strategies for the early detection of lung cancer are urgently sought. Total circulating free DNA (cfDNA) and tumour-derived circulating tumour DNA (ctDNA) are emerging as important biomarkers within a 'liquid biopsy' for monitoring human disease progression and response to therapy. Owing to the late clinical diagnosis of lung adenocarcinoma, the potential for cfDNA and ctDNA as early detection biomarkers remains unexplored. Here, using a Cre-regulated genetically engineered mouse model of lung adenocarcinoma development, driven by Kras
Identifiants
pubmed: 30760495
pii: 12/2/dmm036863
doi: 10.1242/dmm.036863
pmc: PMC6398498
pii:
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
Cre recombinase
EC 2.7.7.-
Integrases
EC 2.7.7.-
Hras protein, mouse
EC 3.6.5.2
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Subventions
Organisme : Cancer Research UK
ID : 13462
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00025/3
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1203/1
Pays : United Kingdom
Organisme : Pancreatic Cancer UK
ID : 2013 RIF - SHAW
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U132674518
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 13083
Pays : United Kingdom
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
Références
Nature. 2013 Sep 19;501(7467):338-45
pubmed: 24048066
Nature. 2012 Jun 28;486(7404):537-40
pubmed: 22722843
Cancer Res. 1977 Mar;37(3):646-50
pubmed: 837366
Cancer Res. 2004 Apr 1;64(7):2307-16
pubmed: 15059877
Blood. 2017 Jul 27;130(4):514-526
pubmed: 28550040
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
pubmed: 12824337
Nat Med. 2014 May;20(5):548-54
pubmed: 24705333
C R Seances Soc Biol Fil. 1948 Feb;142(3-4):241-3
pubmed: 18875018
J Thorac Imaging. 2015 Mar;30(2):130-8
pubmed: 25658477
Dis Markers. 2005;21(3):105-20
pubmed: 16276004
EMBO Mol Med. 2015 Jul 16;7(9):1119-37
pubmed: 26183450
Mol Cell Biol. 2007 Aug;27(16):5921-32
pubmed: 17562862
J Pathol. 2014 Mar;232(4):449-57
pubmed: 24307564
Mol Cell Biochem. 2010 May;338(1-2):263-9
pubmed: 20049629
Cancer Res. 2005 Nov 15;65(22):10280-8
pubmed: 16288016
N Engl J Med. 2013 May 23;368(21):1980-91
pubmed: 23697514
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9209-14
pubmed: 11481484
Cancer Discov. 2017 Dec;7(12):1394-1403
pubmed: 28899864
Genes Dev. 2012 Sep 1;26(17):1945-58
pubmed: 22892241
Breast Cancer Res Treat. 2014 Jul;146(1):163-74
pubmed: 24838941
Nat Rev Cancer. 2011 Jun;11(6):426-37
pubmed: 21562580
N Engl J Med. 2017 Jun 1;376(22):2109-2121
pubmed: 28445112
Sci Transl Med. 2015 Aug 26;7(302):302ra133
pubmed: 26311728
Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4952-7
pubmed: 24586047
Comp Med. 2013;63(6):482-90
pubmed: 24326223
Cancer Cell. 2003 Aug;4(2):111-20
pubmed: 12957286
Eur J Cancer Clin Oncol. 1987 Jun;23(6):707-12
pubmed: 3653190
Biotechniques. 2003 Nov;35(5):914-6, 918, 920
pubmed: 14628662
Nature. 2017 Apr 26;545(7655):446-451
pubmed: 28445469
Clin Cancer Res. 2005 Jul 1;11(13 Pt 2):4999s-5003s
pubmed: 16000603
Int J Radiat Oncol Biol Phys. 2010 Mar 15;76(4):973-7
pubmed: 20206017
Curr Protoc Pharmacol. 2015 Sep 01;70:14.35.1-14.35.16
pubmed: 26331885
Genes Dev. 2001 Dec 15;15(24):3243-8
pubmed: 11751630
Oncotarget. 2017 Apr 24;8(33):54537-54547
pubmed: 28903362