Cerebellar ataxia disease-associated Snx14 promotes lipid droplet growth at ER-droplet contacts.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
21
08
2018
revised:
29
12
2018
accepted:
28
01
2019
pubmed:
16
2
2019
medline:
14
4
2020
entrez:
16
2
2019
Statut:
ppublish
Résumé
Lipid droplets (LDs) are nutrient reservoirs used by cells to maintain homeostasis. Nascent droplets form on the endoplasmic reticulum (ER) and grow following an influx of exogenous fatty acids (FAs). The budding of LDs requires extensive ER-LD crosstalk, but how this is regulated remains poorly understood. Here, we show that sorting nexin protein Snx14, an ER-resident protein associated with the cerebellar ataxia SCAR20, localizes to ER-LD contacts following FA treatment, where it promotes LD maturation. Using proximity-based APEX technology and topological dissection, we show that Snx14 accumulates specifically at ER-LD contacts independently of Seipin, where it remains ER-anchored and binds LDs in trans.
Identifiants
pubmed: 30765438
pii: jcb.201808133
doi: 10.1083/jcb.201808133
pmc: PMC6446855
doi:
Substances chimiques
BSCL2 protein, human
0
Fatty Acids
0
GTP-Binding Protein gamma Subunits
0
SNX14 protein, human
0
Sorting Nexins
0
Oleic Acid
2UMI9U37CP
Coenzyme A Ligases
EC 6.2.1.-
long-chain-fatty-acid-CoA ligase
EC 6.2.1.3
Banques de données
PIR
['616354']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1335-1351Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM119768
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
© 2019 Datta et al.
Références
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3077-82
pubmed: 12629214
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
Cell Mol Life Sci. 2006 Jun;63(12):1355-69
pubmed: 16649142
Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20890-5
pubmed: 18093937
J Cell Biol. 2008 Feb 11;180(3):473-82
pubmed: 18250201
J Cell Sci. 2009 Mar 15;122(Pt 6):749-52
pubmed: 19261844
Cold Spring Harb Perspect Biol. 2011 Mar 01;3(3):null
pubmed: 21421923
J Lipid Res. 2011 Sep;52(9):1760-4
pubmed: 21653930
J Cell Biol. 2012 Sep 3;198(5):895-911
pubmed: 22927462
Nat Biotechnol. 2012 Nov;30(11):1143-8
pubmed: 23086203
Nat Protoc. 2013 Jan;8(1):43-51
pubmed: 23222457
Dev Cell. 2013 Feb 25;24(4):384-99
pubmed: 23415954
J Cell Biol. 2013 Dec 23;203(6):985-1001
pubmed: 24368806
Elife. 2014;3:e01607
pubmed: 24497546
J Biol Chem. 2014 Oct 10;289(41):28554-68
pubmed: 25148684
Nat Methods. 2015 Jan;12(1):51-4
pubmed: 25419960
Am J Hum Genet. 2014 Nov 6;95(5):611-21
pubmed: 25439728
Mol Biol Cell. 2015 Feb 15;26(4):726-39
pubmed: 25540432
Elife. 2016 Aug 26;5:null
pubmed: 27564575
Cell Rep. 2016 Nov 1;17(6):1546-1559
pubmed: 27806294
EMBO J. 2016 Dec 15;35(24):2699-2716
pubmed: 27879284
Eur J Med Genet. 2017 Feb;60(2):118-123
pubmed: 27913285
J Cell Biol. 2017 Oct 2;216(10):3199-3217
pubmed: 28801319
EMBO Rep. 2018 Jan;19(1):57-72
pubmed: 29146766
Dev Cell. 2018 Jan 08;44(1):73-86.e4
pubmed: 29316443
J Cell Biol. 2018 Mar 5;217(3):975-995
pubmed: 29367353
Hum Mol Genet. 2018 Jun 1;27(11):1927-1940
pubmed: 29635513
Dev Cell. 2018 Oct 22;47(2):248-256.e4
pubmed: 30293840
J Cell Biol. 2018 Dec 3;217(12):4080-4091
pubmed: 30327422
J Cell Biol. 2019 Apr 1;218(4):1319-1334
pubmed: 30808705