Glycyl-tRNA synthetase from Nanoarchaeum equitans: The first crystal structure of archaeal GlyRS and analysis of its tRNA glycylation.

Amino Acid Sequence Archaeal Proteins / chemistry Crystallography, X-Ray Glycine-tRNA Ligase / chemistry Models, Molecular Nanoarchaeota / chemistry Protein Conformation RNA, Transfer / chemistry Sequence Alignment
Acceptor-stem recognition Aminoacylation Crystal structure Glycyl-tRNA synthetase Nanoarchaeum equitans tRNA

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
02 04 2019
Historique:
received: 23 01 2019
accepted: 31 01 2019
pubmed: 18 2 2019
medline: 24 12 2019
entrez: 18 2 2019
Statut: ppublish

Résumé

This study reports the X-ray crystallographic structure of the glycyl-tRNA synthetase (GlyRS) of Nanoarchaeum equitans - a hyperthermophilic archaeal species. This is the first archaeal GlyRS crystal structure elucidated. The GlyRS comprises an N-terminal catalytic domain and a C-terminal anticodon-binding domain with a long β-sheet inserted between these domains. An unmodified transcript of the wild-type N. equitans tRNA

Identifiants

DOI: 10.1016/j.bbrc.2019.01.142 PMID: 30771900
pubmed: 30771900
pii: S0006-291X(19)30176-7
doi: 10.1016/j.bbrc.2019.01.142
pii:
doi:

Substances chimiques

Archaeal Proteins 0
RNA, Transfer 9014-25-9
Glycine-tRNA Ligase EC 6.1.1.14

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

228-233

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Alma Fujisawa (A)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Risako Toki (R)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Hideaki Miyake (H)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Tomoko Shoji (T)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Hiromi Doi (H)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Hiromi Hayashi (H)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Rina Hanabusa (R)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Hiromi Mutsuro-Aoki (H)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Takuya Umehara (T)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Tadashi Ando (T)

Department of Applied Electronics, Tokyo University of Science, Katsushika-ku, Tokyo 125-8585, Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.

Hiroki Noguchi (H)

Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan; Laboratory of Biomolecular Modelling and Design, Department of Chemistry, KU Leuven, Celestijnenlaan 200G-bus2403, 3001 Leuven, Belgium.

Arnout Voet (A)

Laboratory of Biomolecular Modelling and Design, Department of Chemistry, KU Leuven, Celestijnenlaan 200G-bus2403, 3001 Leuven, Belgium.

Sam-Yong Park (SY)

Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan.

Koji Tamura (K)

Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: koji@rs.tus.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'archée › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-oxygen ligases › Amino acyl-tRNA synthetases › Glycine-tRNA ligase › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Archéobactéries › Nanoarchaeota › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › Glycyl-tRNA synthetase from Nanoarchaeum...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Glycyl-tRNA synthetase from Nanoarchaeum...