T lymphocyte phenotype of contact-allergic patients: experience with nickel and p-phenylenediamine.
CD4-CD8 Ratio
Case-Control Studies
Cell Proliferation
Coloring Agents
/ adverse effects
Dermatitis, Allergic Contact
/ etiology
Disinfectants
/ adverse effects
Humans
Immunologic Memory
/ immunology
Lymphocyte Activation
Nickel
/ adverse effects
Oligosaccharides
/ immunology
Patch Tests
Phenotype
Phenylenediamines
/ adverse effects
Sialyl Lewis X Antigen
/ analogs & derivatives
T-Lymphocytes
/ immunology
T-Lymphocytes, Regulatory
/ immunology
Thiazoles
/ adverse effects
CD4
CD8
RRID:SCR_001905
T cell phenotype
inverted CD4/CD8, skin-homing CLA
lymphocyte transformation test
Journal
Contact dermatitis
ISSN: 1600-0536
Titre abrégé: Contact Dermatitis
Pays: England
ID NLM: 7604950
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
17
12
2018
revised:
11
02
2019
accepted:
14
02
2019
pubmed:
20
2
2019
medline:
24
12
2019
entrez:
20
2
2019
Statut:
ppublish
Résumé
There is considerable interest in understanding the immunological variables that have the greatest influence on the effectiveness of sensitization by contact allergens, particularly in the context of developing new paradigms for risk assessment of novel compounds. To examine the relationship between patch test score for three different contact allergens and the characteristics of T cell responses. A total of 192 patients with confirmed nickel, p-phenylenediamine (PPD) or methylisothiazolinone (MI) allergy were recruited from the Contact Dermatitis Investigation Unit at Salford Royal Hospital. Severity of allergy was scored by the use of patch testing, peripheral blood lymphocytes were characterized for T cell phenotype by flow cytometry, and proliferative activity was characterized by radiolabelled thymidine incorporation. Comparisons were drawn with buffy coat samples from healthy volunteers. Patch test positivity for nickel, PPD and MI was associated with changes in the phenotype of peripheral blood T cells: increases in naïve cells, decreases in regulatory T cell frequency and the CD4 This increased understanding of the characteristics of the T cell responses to contact allergens may provide parameters with which to better measure health risks associated with skin sensitization.
Sections du résumé
BACKGROUND
BACKGROUND
There is considerable interest in understanding the immunological variables that have the greatest influence on the effectiveness of sensitization by contact allergens, particularly in the context of developing new paradigms for risk assessment of novel compounds.
OBJECTIVES
OBJECTIVE
To examine the relationship between patch test score for three different contact allergens and the characteristics of T cell responses.
METHODS
METHODS
A total of 192 patients with confirmed nickel, p-phenylenediamine (PPD) or methylisothiazolinone (MI) allergy were recruited from the Contact Dermatitis Investigation Unit at Salford Royal Hospital. Severity of allergy was scored by the use of patch testing, peripheral blood lymphocytes were characterized for T cell phenotype by flow cytometry, and proliferative activity was characterized by radiolabelled thymidine incorporation. Comparisons were drawn with buffy coat samples from healthy volunteers.
RESULTS
RESULTS
Patch test positivity for nickel, PPD and MI was associated with changes in the phenotype of peripheral blood T cells: increases in naïve cells, decreases in regulatory T cell frequency and the CD4
CONCLUSIONS
CONCLUSIONS
This increased understanding of the characteristics of the T cell responses to contact allergens may provide parameters with which to better measure health risks associated with skin sensitization.
Substances chimiques
6-sulfo sialyl Lewis X
0
Coloring Agents
0
Disinfectants
0
Oligosaccharides
0
Phenylenediamines
0
Sialyl Lewis X Antigen
0
Thiazoles
0
2-methyl-4-isothiazolin-3-one
229D0E1QFA
Nickel
7OV03QG267
4-phenylenediamine
U770QIT64J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-53Subventions
Organisme : Medical Research Council
ID : MR/L011840/1
Pays : United Kingdom
Informations de copyright
© 2019 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd.