Modes of Accessing Bicarbonate for the Regulation of Membrane Guanylate Cyclase (ROS-GC) in Retinal Rods and Cones.


Journal

eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362

Informations de publication

Date de publication:
Historique:
received: 11 10 2018
revised: 20 12 2018
accepted: 15 01 2019
entrez: 21 2 2019
pubmed: 21 2 2019
medline: 19 4 2019
Statut: epublish

Résumé

The membrane guanylate cyclase, ROS-GC, that synthesizes cyclic GMP for use as a second messenger for visual transduction in retinal rods and cones, is stimulated by bicarbonate. Bicarbonate acts directly on ROS-GC1, because it enhanced the enzymatic activity of a purified, recombinant fragment of bovine ROS-GC1 consisting solely of the core catalytic domain. Moreover, recombinant ROS-GC1 proved to be a true sensor of bicarbonate, rather than a sensor for CO

Identifiants

pubmed: 30783616
doi: 10.1523/ENEURO.0393-18.2019
pii: eN-NWR-0393-18
pmc: PMC6378327
pii:
doi:

Substances chimiques

Bicarbonates 0
Carbonic Anhydrase Inhibitors 0
Receptors, Cell Surface 0
Recombinant Proteins 0
Carbon Dioxide 142M471B3J
Carbonic Anhydrases EC 4.2.1.1
Guanylate Cyclase EC 4.6.1.2
Cyclic GMP H2D2X058MU
Acetazolamide O3FX965V0I

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NEI NIH HHS
ID : R01 EY023980
Pays : United States

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Auteurs

Clint L Makino (CL)

Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118.

Teresa Duda (T)

Research Divisions of Biochemistry and Molecular Biology, Unit of Regulatory and Molecular Biology, Salus University, Elkins Park, PA 19027.

Alexandre Pertzev (A)

Research Divisions of Biochemistry and Molecular Biology, Unit of Regulatory and Molecular Biology, Salus University, Elkins Park, PA 19027.

Tomoki Isayama (T)

Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118.

Polina Geva (P)

Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118.

Michael A Sandberg (MA)

Department of Ophthalmology, Harvard Medical School, Boston, MA 02114.

Rameshwar K Sharma (RK)

Research Divisions of Biochemistry and Molecular Biology, Unit of Regulatory and Molecular Biology, Salus University, Elkins Park, PA 19027.

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Classifications MeSH