DNA binding adaptors to assemble proteins of interest on DNA scaffold.
Atomic force microscopy
Cross-linking
DNA binding proteins
DNA nanostructures
DNA origami
Enzyme cascade
Orthogonal reactions
Self-ligating protein tag
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
21
2
2019
pubmed:
21
2
2019
medline:
26
11
2019
Statut:
ppublish
Résumé
DNA nanostructures serve as the ideal scaffolds to assemble materials of interest. Among these, proteins are of particularly interesting class of molecules to assemble because of their huge functional variability. Sequence-specific DNA binding proteins have been applied as adaptors to stably locate the fused proteins at defined positions of DNA scaffold in high loading yields. The strategy allows to control the number of enzyme molecules and to maintain the catalytic activity. By fusing a chemoselective self-ligating protein tag to the DNA binding protein, the modular adaptors formed covalent bonds at respective sequences on DNA scaffold with fast reaction kinetics. Application of a set of orthogonal modular adaptors enables spatial organization of multiple types of enzymes.
Identifiants
pubmed: 30784406
pii: S0076-6879(18)30510-X
doi: 10.1016/bs.mie.2018.12.014
pii:
doi:
Substances chimiques
Cross-Linking Reagents
0
DNA-Binding Proteins
0
Enzymes, Immobilized
0
Recombinant Fusion Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
287-322Informations de copyright
© 2019 Elsevier Inc. All rights reserved.