Tricarboxylic acid metabolon.
Cross-linking mass spectrometry
Interactomics
Metabolon
Substrate channeling
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
21
2
2019
pubmed:
21
2
2019
medline:
26
11
2019
Statut:
ppublish
Résumé
This chapter focuses on the experimental protocols used to determine the structure of the TCA metabolon. Since the TCA metabolon is quite large and cannot be crystallized, X-ray crystallography and NMR-based structural biology techniques cannot be utilized. This chapter will include a discussion of the interactomics technique of cross-linking mass spectrometry used for determining the TCA metabolon structure, including detailed procedures and the resulting metabolon structures. It will conclude with a discussion of the lessons learned from the structure of the TCA metabolon that are important to the field of metabolism.
Identifiants
pubmed: 30784407
pii: S0076-6879(18)30498-1
doi: 10.1016/bs.mie.2018.12.002
pii:
doi:
Substances chimiques
Tricarboxylic Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-43Informations de copyright
© 2019 Elsevier Inc. All rights reserved.