Molecular and functional insights into Transient Receptor Potential Vanilloid 1 (TRPV1) in bull spermatozoa.

Acrosome Reaction Animals Arachidonic Acids / pharmacology Calcium Channel Blockers / pharmacology Capsaicin / analogs & derivatives Cattle Endocannabinoids / pharmacology Male Polyunsaturated Alkamides / pharmacology Sperm Capacitation Sperm Motility Spermatozoa / metabolism TRPV Cation Channels / metabolism
AEA Bull Capacitation Cp Hyperactive motility Progressive sperm motility Spermatozoa TRPV1

Journal

Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510

Informations de publication

Date de publication:
01 Apr 2019
Historique:
received: 01 07 2018
revised: 30 01 2019
accepted: 31 01 2019
pubmed: 21 2 2019
medline: 23 7 2019
entrez: 21 2 2019
Statut: ppublish

Résumé

In view of the limited information available on functional significance of TRPV1 in regulating sperm functions, present study was undertaken on bull spermatozoa. Sixty four ejaculates were collected from four Hariana bulls and were used for molecular and functional characterisation of TRPV1. Immunoblotting using TRPV1 specific antibody revealed the presence of a single band of 104 kDa corresponding to TRPV1 in Hariana bull spermatozoa. Indirect immuno fluorescence revealed positive immune-reactivity to TRPV1 at acrosomal, pre-acrosomal, post acrosomal and flagellar regions of spermatozoa. Based on the results of pilot study dose-response analysis, doses of anandamide (AEA; 0.3 μM) and capsazepine (Cp; 10 μM) were selected for further studies. Three groups of semen samples (control 100 μL diluted semen having 1 × 10

Identifiants

DOI: 10.1016/j.theriogenology.2019.01.029 PMID: 30784807
pubmed: 30784807
pii: S0093-691X(18)30435-7
doi: 10.1016/j.theriogenology.2019.01.029
pii:
doi:

Substances chimiques

Arachidonic Acids 0
Calcium Channel Blockers 0
Endocannabinoids 0
Polyunsaturated Alkamides 0
TRPV Cation Channels 0
capsazepine LFW48MY844
Capsaicin S07O44R1ZM
anandamide UR5G69TJKH

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

207-217

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Akshay Kumar (A)

Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Abhishek Kumar Mishra (AK)

College of Biotechnology, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Vijay Singh (V)

Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Sarvajeet Yadav (S)

Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Atul Saxena (A)

Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Satish Kumar Garg (SK)

Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India.

Dilip Kumar Swain (DK)

Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India. Electronic address: dilip_swain@yahoo.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Fécondation › Interaction sperme-ovule › Réaction acrosomique › Molecular and functional insights into...
questionsmedicales.fr › Eucaryotes › Animaux › Molecular and functional insights into...
questionsmedicales.fr › Lipides › Acides gras › Acides gras insaturés › Acides gras indispensables › Acides arachidoniques › Molecular and functional insights into...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents cardiovasculaires › Inhibiteurs des canaux calciques › Molecular and functional insights into...
questionsmedicales.fr › Lipides › Acides gras › Acides gras insaturés › Acides gras monoinsaturés › Capsaïcine › Molecular and functional insights into...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Molecular and functional insights into...
questionsmedicales.fr › Lipides › Acides gras › Endocannabinoïdes › Molecular and functional insights into...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcynes › Amides gras polyinsaturés N-alkylés › Molecular and functional insights into...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Fécondation › Capacitation des spermatozoïdes › Molecular and functional insights into...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mobilité des spermatozoïdes › Molecular and functional insights into...
questionsmedicales.fr › Cellules › Cellules germinales › Spermatozoïdes › Molecular and functional insights into...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Canaux cationiques TRPV › Molecular and functional insights into...