Comparison of the diagnostic value of symmetric dimethylarginine, cystatin C, and creatinine for detection of decreased glomerular filtration rate in dogs.


Journal

Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660

Informations de publication

Date de publication:
Mar 2019
Historique:
received: 22 01 2018
accepted: 23 01 2019
pubmed: 23 2 2019
medline: 30 6 2019
entrez: 22 2 2019
Statut: ppublish

Résumé

Early detection of decreased glomerular filtration rate (GFR) in dogs is challenging. Current methods are insensitive and new biomarkers are required. To compare overall diagnostic performance of serum symmetric dimethylarginine (SDMA) and serum cystatin C to serum creatinine, for detection of decreased GFR in clinically stable dogs, with or without chronic kidney disease (CKD). Ninety-seven client-owned dogs: 67 dogs with a diagnosis or suspicion of CKD and 30 healthy dogs were prospectively included. Prospective diagnostic accuracy study. All dogs underwent physical examination, systemic arterial blood pressure measurement, urinalysis, hematology and blood biochemistry analysis, cardiac and urinary ultrasound examinations, and scintigraphy for estimation of glomerular filtration rate (mGFR). Frozen serum was used for batch analysis of SDMA and cystatin C. The area under the curve of creatinine, SDMA, and cystatin C for detection of an mGFR <30.8 mL/min/L was 0.98 (95% confidence interval [CI], 0.93-1.0), 0.96 (95% CI, 0.91-0.99), and 0.87 (95% CI, 0.79-0.93), respectively. The sensitivity of both creatinine and SDMA at their prespecified cutoffs (115 μmol/L [1.3 mg/dL] and 14 μg/dL) for detection of an abnormal mGFR was 90%. The specificity was 90% for creatinine and 87% for SDMA. When adjusting the cutoff for cystatin C to correspond to a diagnostic sensitivity of 90% (0.49 mg/L), specificity was lower (72%) than that of creatinine and SDMA. Overall diagnostic performance of creatinine and SDMA for detection of decreased mGFR was similar. Overall diagnostic performance of cystatin C was inferior to both creatinine and SDMA.

Sections du résumé

BACKGROUND BACKGROUND
Early detection of decreased glomerular filtration rate (GFR) in dogs is challenging. Current methods are insensitive and new biomarkers are required.
OBJECTIVE OBJECTIVE
To compare overall diagnostic performance of serum symmetric dimethylarginine (SDMA) and serum cystatin C to serum creatinine, for detection of decreased GFR in clinically stable dogs, with or without chronic kidney disease (CKD).
ANIMALS METHODS
Ninety-seven client-owned dogs: 67 dogs with a diagnosis or suspicion of CKD and 30 healthy dogs were prospectively included.
METHODS METHODS
Prospective diagnostic accuracy study. All dogs underwent physical examination, systemic arterial blood pressure measurement, urinalysis, hematology and blood biochemistry analysis, cardiac and urinary ultrasound examinations, and scintigraphy for estimation of glomerular filtration rate (mGFR). Frozen serum was used for batch analysis of SDMA and cystatin C.
RESULTS RESULTS
The area under the curve of creatinine, SDMA, and cystatin C for detection of an mGFR <30.8 mL/min/L was 0.98 (95% confidence interval [CI], 0.93-1.0), 0.96 (95% CI, 0.91-0.99), and 0.87 (95% CI, 0.79-0.93), respectively. The sensitivity of both creatinine and SDMA at their prespecified cutoffs (115 μmol/L [1.3 mg/dL] and 14 μg/dL) for detection of an abnormal mGFR was 90%. The specificity was 90% for creatinine and 87% for SDMA. When adjusting the cutoff for cystatin C to correspond to a diagnostic sensitivity of 90% (0.49 mg/L), specificity was lower (72%) than that of creatinine and SDMA.
CONCLUSIONS AND CLINICAL IMPORTANCE CONCLUSIONS
Overall diagnostic performance of creatinine and SDMA for detection of decreased mGFR was similar. Overall diagnostic performance of cystatin C was inferior to both creatinine and SDMA.

Identifiants

pubmed: 30791142
doi: 10.1111/jvim.15445
pmc: PMC6430914
doi:

Substances chimiques

Biomarkers 0
Cystatin C 0
symmetric dimethylarginine 49787G1ULV
Arginine 94ZLA3W45F
Creatinine AYI8EX34EU

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

630-639

Subventions

Organisme : Agria/SKK Research Foundation
Organisme : Michael Forsgren Foundation
Organisme : Thure F och Karin Forsbergs Stiftelse

Informations de copyright

© 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

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Auteurs

Lena Pelander (L)

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Jens Häggström (J)

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Anders Larsson (A)

Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

Harriet Syme (H)

Department of Clinical Science and Services, The Royal Veterinary College, Hertfordshire, United Kingdom.

Jonathan Elliott (J)

Department of Comparative Biomedical Sciences, The Royal Veterinary College, London, United Kingdom.

Reidun Heiene (R)

ABC Dyreklinikk Lillehammer AS, Hamarvegen 68A, 26 13 Lillehammer, Norway.

Ingrid Ljungvall (I)

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

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Classifications MeSH