Approaches for Studying Ribosome Specialization.

Chromatography, Liquid Models, Molecular Proteins / chemistry RNA, Ribosomal / chemistry Ribosomes / chemistry Tandem Mass Spectrometry

Journal

Trends in biochemical sciences
ISSN: 0968-0004
Titre abrégé: Trends Biochem Sci
Pays: England
ID NLM: 7610674

Informations de publication

Date de publication:
05 2019
Historique:
received: 23 01 2019
accepted: 23 01 2019
pubmed: 23 2 2019
medline: 7 8 2019
entrez: 23 2 2019
Statut: ppublish

Résumé

Contrary to the textbook model, recent measurements demonstrated unexpected diversity in ribosomal composition that likely enables specialized translational functions. Methods based on liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) enable direct quantification of ribosomal proteins with high specificity, accuracy, and throughput. LC-MS/MS can be 'top-down', analyzing intact proteins, or more commonly 'bottom-up', where proteins are digested to peptides prior to analysis. Changes to rRNA can be examined using either LC-MS/MS or sequencing-based approaches. The regulation of protein synthesis by specialized ribosomes can be examined by multiple methods. These include the popular 'Ribo-Seq' method for analyzing ribosome density on a given mRNA, as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor protein synthesis and degradation.

Identifiants

DOI: 10.1016/j.tibs.2019.01.008 PMID: 30792028 PMC: PMC6698392
pubmed: 30792028
pii: S0968-0004(19)30017-9
doi: 10.1016/j.tibs.2019.01.008
pmc: PMC6698392
mid: NIHMS1045735
pii:
doi:

Substances chimiques

Proteins 0
RNA, Ribosomal 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Review

Langues

eng

Pagination

478-479

Subventions

Organisme : NIGMS NIH HHS
ID : DP2 GM123497
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM128802
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Références

J Am Chem Soc. 2014 Feb 5;136(5):2058-69
pubmed: 24422502
Nat Rev Genet. 2014 Mar;15(3):205-13
pubmed: 24468696
Cell Rep. 2015 Nov 3;13(5):865-73
pubmed: 26565899
J Am Soc Mass Spectrom. 2017 Dec;28(12):2614-2634
pubmed: 28875426
Nat Methods. 2018 Mar;15(3):201-206
pubmed: 29334379
Mol Cell Proteomics. 2018 May;17(5):974-992
pubmed: 29414762
Sci Adv. 2018 Feb 28;4(2):eaao0665
pubmed: 29503865
Mol Cell Proteomics. 2019 Jan;18(1):162-168
pubmed: 30282776
Trends Biochem Sci. 2019 Feb;44(2):95-109
pubmed: 30473427

Auteurs

Edward Emmott (E)

Department of Bioengineering, Northeastern University, Boston, MA, USA; Barnett Institute for Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.

Marko Jovanovic (M)

Department of Biological Sciences, Columbia University, New York, NY, USA.

Nikolai Slavov (N)

Department of Bioengineering, Northeastern University, Boston, MA, USA; Barnett Institute for Chemical and Biological Analysis, Northeastern University, Boston, MA, USA; Department of Biology, Northeastern University, Boston, MA, USA. Electronic address: nslavov@alum.mit.edu.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Approaches for Studying Ribosome Specialization.
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Approaches for Studying Ribosome Specialization.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Approaches for Studying Ribosome Specialization.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › Approaches for Studying Ribosome Specialization.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Approaches for Studying Ribosome Specialization.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Approaches for Studying Ribosome Specialization.