Biosynthesis of novel lactate-based polymers containing medium-chain-length 3-hydroxyalkanoates by recombinant Escherichia coli strains from glucose.

Acyltransferases / genetics Alcohol Oxidoreductases / genetics DNA, Recombinant / genetics Escherichia coli / genetics Glucose / metabolism Lactic Acid / chemistry Polymers / chemistry Pseudomonas / genetics
Biodegradable plastic Biopolymer Poly(lactic acid) Polyhydroxyalkanoate d-Lactate dehydrogenase

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 18 12 2018
revised: 10 01 2019
accepted: 15 01 2019
pubmed: 26 2 2019
medline: 12 10 2019
entrez: 26 2 2019
Statut: ppublish

Résumé

Novel lactate (LA)-based polymers containing medium-chain-length 3-hydroxyalkanoates (MCL-3HA) were produced in fadR-deficient Escherichia coli strains from glucose as the sole carbon source. The genes encoding LA and 3-hydroxybutyrate (3HB) monomers supplying enzymes [propionyl-CoA transferase (PCT), d-lactate dehydrogenase (D-LDH), β-ketothiolase (PhaA), and NADPH-dependent acetoacetyl-CoA reductase (PhaB)], MCL-3HA monomers supplying enzymes [(R)-3-hydroxyacyl-ACP thioesterase (PhaG) and (R)-3-hydroxyacyl (3HA)-CoA ligase] via fatty acid biosynthesis pathway, and modified polyhydroxyalkanoate (PHA) synthase [PhaC1(STQK)] of Pseudomonas sp. 61-3 were introduced into E. coli LS5218. This resulted in the synthesis of a novel LA-based copolymer, P(LA-co-3HB-co-3HA).

Identifiants

DOI: 10.1016/j.jbiosc.2019.01.009 PMID: 30799088
pubmed: 30799088
pii: S1389-1723(18)31170-8
doi: 10.1016/j.jbiosc.2019.01.009
pii:
doi:

Substances chimiques

DNA, Recombinant 0
Polymers 0
Lactic Acid 33X04XA5AT
Alcohol Oxidoreductases EC 1.1.-
acetoacetyl-CoA reductase EC 1.1.1.36
Acyltransferases EC 2.3.-
poly(3-hydroxyalkanoic acid) synthase EC 2.3.1.-
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

191-197

Informations de copyright

Copyright © 2019 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Saki Goto (S)

Department of Food and Health Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, Kumamoto 862-8502, Japan.

Ayaka Hokamura (A)

Department of Food and Health Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, Kumamoto 862-8502, Japan.

Hideki Shiratsuchi (H)

Department of Food and Health Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, Kumamoto 862-8502, Japan.

Seiichi Taguchi (S)

Department of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuraoka, Setagaya-Ku, Tokyo 156-8502, Japan.

Ken'ichiro Matsumoto (K)

Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan.

Hideki Abe (H)

Bioplastic Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

Kenji Tanaka (K)

Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.

Hiromi Matsusaki (H)

Department of Food and Health Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, Kumamoto 862-8502, Japan. Electronic address: matusaki@pu-kumamoto.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN recombiné › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Hydroxyacides › Lactates › Acide lactique › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biosynthesis of novel lactate-based polymers...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Biosynthesis of novel lactate-based polymers...