System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection.
5' Untranslated Regions
Binding Sites
Epithelial Cells
/ metabolism
Exoribonucleases
/ genetics
Female
Gene Expression Profiling
/ methods
Gene Expression Regulation, Viral
HEK293 Cells
HeLa Cells
Host-Pathogen Interactions
Humans
Microtubule-Associated Proteins
/ genetics
Protein Binding
RNA, Viral
/ genetics
RNA-Binding Proteins
/ genetics
Ribonucleoproteins, Small Nuclear
/ genetics
SMN Complex Proteins
Sindbis Virus
/ genetics
Transcriptome
Uterine Cervical Neoplasms
/ genetics
Virus Replication
GEMIN5
RNA-binding protein
RNA-interactome capture
Sindbis
TRIM25
XRN1
alphavirus
host-virus interaction
protein-RNA interaction
virus infection
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
04 04 2019
04 04 2019
Historique:
received:
18
06
2018
revised:
18
12
2018
accepted:
11
01
2019
pubmed:
26
2
2019
medline:
16
7
2019
entrez:
26
2
2019
Statut:
ppublish
Résumé
The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA-interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these questions, we developed "comparative RIC" and applied it to cells challenged with an RNA virus called sindbis (SINV). Over 200 RBPs display differential interaction with RNA upon SINV infection. These alterations are mainly driven by the loss of cellular mRNAs and the emergence of viral RNA. RBPs stimulated by the infection redistribute to viral replication factories and regulate the capacity of the virus to infect. For example, ablation of XRN1 causes cells to be refractory to SINV, while GEMIN5 moonlights as a regulator of SINV gene expression. In summary, RNA availability controls RBP localization and function in SINV-infected cells.
Identifiants
pubmed: 30799147
pii: S1097-2765(19)30037-1
doi: 10.1016/j.molcel.2019.01.017
pmc: PMC6458987
pii:
doi:
Substances chimiques
5' Untranslated Regions
0
GEMIN5 protein, human
0
Microtubule-Associated Proteins
0
RNA, Viral
0
RNA-Binding Proteins
0
Ribonucleoproteins, Small Nuclear
0
SMN Complex Proteins
0
Exoribonucleases
EC 3.1.-
XRN1 protein, human
EC 3.1.13.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
196-211.e11Subventions
Organisme : Medical Research Council
ID : MR/R021562/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L019434/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209412/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 092076
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/10
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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