Molecular Epidemiology of Human Astrovirus in Stool Samples From Patients With Acute Gastroenteritis in Korea, 2013-2017.
Adolescent
Adult
Aged
Aged, 80 and over
Astroviridae
/ classification
Astroviridae Infections
/ diagnosis
Capsid Proteins
/ genetics
Child
Child, Preschool
Feces
/ virology
Gastroenteritis
/ diagnosis
Genotype
Humans
Infant
Middle Aged
Multiplex Polymerase Chain Reaction
Phylogeny
RNA, Viral
/ chemistry
Republic of Korea
/ epidemiology
Young Adult
Astrovirus
Epidemiology
Genotype
Korea
PCR
Journal
Annals of laboratory medicine
ISSN: 2234-3814
Titre abrégé: Ann Lab Med
Pays: Korea (South)
ID NLM: 101571172
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
04
11
2018
revised:
24
12
2018
accepted:
04
02
2019
entrez:
28
2
2019
pubmed:
28
2
2019
medline:
4
6
2019
Statut:
ppublish
Résumé
Human astroviruses (HAstVs) are one of the major causes of acute gastroenteritis. Classic HAstVs can be classified into eight genotypes. We investigated the positive rate of HAstVs and the distribution of HAstV genotypes in strains isolated from patients with acute gastroenteritis in Hwaseong, Korea, in 2013-2017. Between November 2013 and December 2017, 3,519 stool samples were collected from patients with symptoms of acute gastroenteritis and tested for HAstV using multiplex PCR. For HAstV-positive samples, the The positive rate of HAstV was 1.9% (67 samples). HAstV Type 1 was the most prevalent genotype (82.4%), and Types 4, 5, and 8 were also detected. Infection occurred year-round, with no distinct seasonal variation. Infection occurred at nearly all ages (55 days-81 years; median: 3 years), and the positive rate was substantially higher in children younger than five years. Phylogenetic analysis revealed three distinct clades of HAstV Type 1 according to the collection time. Our results provide recent epidemiological data on HAstVs in Korea between 2013 and 2017. The finding of three distinct clades of HAstV Type 1 according to collection time suggests genetic evolution of HAstVs. These findings can enhance our knowledge on HAstV infection and viral evolution.
Sections du résumé
BACKGROUND
BACKGROUND
Human astroviruses (HAstVs) are one of the major causes of acute gastroenteritis. Classic HAstVs can be classified into eight genotypes. We investigated the positive rate of HAstVs and the distribution of HAstV genotypes in strains isolated from patients with acute gastroenteritis in Hwaseong, Korea, in 2013-2017.
METHODS
METHODS
Between November 2013 and December 2017, 3,519 stool samples were collected from patients with symptoms of acute gastroenteritis and tested for HAstV using multiplex PCR. For HAstV-positive samples, the
RESULTS
RESULTS
The positive rate of HAstV was 1.9% (67 samples). HAstV Type 1 was the most prevalent genotype (82.4%), and Types 4, 5, and 8 were also detected. Infection occurred year-round, with no distinct seasonal variation. Infection occurred at nearly all ages (55 days-81 years; median: 3 years), and the positive rate was substantially higher in children younger than five years. Phylogenetic analysis revealed three distinct clades of HAstV Type 1 according to the collection time.
CONCLUSIONS
CONCLUSIONS
Our results provide recent epidemiological data on HAstVs in Korea between 2013 and 2017. The finding of three distinct clades of HAstV Type 1 according to collection time suggests genetic evolution of HAstVs. These findings can enhance our knowledge on HAstV infection and viral evolution.
Identifiants
pubmed: 30809982
pii: 39.367
doi: 10.3343/alm.2019.39.4.367
pmc: PMC6400717
doi:
Substances chimiques
Capsid Proteins
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
367-372Subventions
Organisme : Hallym University
ID : HURF-2017-30
Pays : Korea
Informations de copyright
© The Korean Society for Laboratory Medicine.
Déclaration de conflit d'intérêts
No potential conflicts of interest relevant to this study are reported.
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