A proline insertion-deletion in the spike glycoprotein fusion peptide of mouse hepatitis virus strongly alters neuropathology.
central proline
demyelination
fusion peptide
fusion protein
membrane fusion
mouse hepatitis virus
neurodegeneration
neuroinflammation
neuropathology
plus-stranded RNA virus
spike glycoprotein
syncytia formation
viral titer
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
17 05 2019
17 05 2019
Historique:
received:
12
06
2018
revised:
24
02
2019
pubmed:
3
3
2019
medline:
18
12
2019
entrez:
3
3
2019
Statut:
ppublish
Résumé
Fusion peptides (FPs) in spike proteins are key players mediating early events in cell-to-cell fusion, vital for intercellular viral spread. A proline residue located at the central FP region has often been suggested to have a distinctive role in this fusion event. The spike glycoprotein from strain RSA59 (PP) of mouse hepatitis virus (MHV) contains two central, consecutive prolines in the FP. Here, we report that deletion of one of these proline residues, resulting in RSA59 (P), significantly affected neural cell syncytia formation and viral titers postinfection
Identifiants
pubmed: 30824541
pii: S0021-9258(20)36364-X
doi: 10.1074/jbc.RA118.004418
pmc: PMC6527167
pii:
doi:
Substances chimiques
Viral Envelope Proteins
0
Proline
9DLQ4CIU6V
Banques de données
PDB
['3JCL']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8064-8087Informations de copyright
© 2019 Singh et al.
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