Vegfa/vegfr2 signaling is necessary for zebrafish islet vessel development, but is dispensable for beta-cell and alpha-cell formation.
Animals
Animals, Genetically Modified
/ genetics
Glucagon-Secreting Cells
/ cytology
Insulin-Secreting Cells
/ cytology
Neovascularization, Physiologic
Signal Transduction
Vascular Endothelial Growth Factor A
/ genetics
Vascular Endothelial Growth Factor Receptor-2
/ genetics
Zebrafish
/ genetics
Zebrafish Proteins
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 03 2019
05 03 2019
Historique:
received:
06
11
2018
accepted:
29
01
2019
entrez:
7
3
2019
pubmed:
7
3
2019
medline:
24
9
2020
Statut:
epublish
Résumé
The mechanisms underlying zebrafish pancreatic islet vascularization have not been well characterized. We sought to determine the angiogenic factors responsible for islet vascularization and assess whether an absence of endothelial cells affects beta-cell and alpha-cell formation. We used a double transgenic zebrafish Tg(fli1:EGFP; insa:tagRFP) to label endothelial cells and beta-cells, respectively. Beta-cells developed adjacent to endothelial cells and by 72 hours post fertilization (hpf) the zebrafish pancreatic islet was highly vascularized. Zebrafish beta-cells express vascular endothelial growth factors (vegf), vegfaa and vegfab. Double knockdown of vegfaa and vegfab or the primary Vegfa receptors (Vegfr2), kdr and kdrl, resulted in vessel deficient islets. While beta-cell and alpha-cell numbers remained unchanged in vessel deficient islets, insulina expression was downregulated relative to controls. Vegfaa/Vegfab-Vegfr2 signaling is necessary for proper islet vessel development, but not for the initial formation of beta-cells and alpha-cells.
Identifiants
pubmed: 30837605
doi: 10.1038/s41598-019-40136-1
pii: 10.1038/s41598-019-40136-1
pmc: PMC6401103
doi:
Substances chimiques
Vascular Endothelial Growth Factor A
0
Vegfaa protein, zebrafish
0
Zebrafish Proteins
0
Vascular Endothelial Growth Factor Receptor-2
EC 2.7.10.1
kdr protein, zebrafish
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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