Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line.
GABA
GAD67-promotor
RRID:AB_10000343
RRID:AB_10562708
RRID:AB_10563568
RRID:AB_10563770
RRID:AB_11180061
RRID:AB_2057032
RRID:AB_2255365
RRID:AB_2307443
RRID:AB_2338013
RRID:AB_2338078
RRID:AB_2338251
RRID:AB_2338680
RRID:AB_2338928
RRID:AB_258984
RRID:AB_310288
RRID:AB_477019
eGFP
interneurons
nucleus endopiriformis
off-target labeling
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
received:
25
01
2019
revised:
04
03
2019
accepted:
04
03
2019
pubmed:
9
3
2019
medline:
6
10
2020
entrez:
9
3
2019
Statut:
ppublish
Résumé
Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor-driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene expression specificity in these animal models is highly recommended to validate cellular identity and to isolate potentially mislabeled cell populations. Here, we report on one such mislabeled neuron population in a widely used transgenic mouse line in which GABAergic somatostatin-expressing interneurons (SOM
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Glutamate Decarboxylase
EC 4.1.1.15
glutamate decarboxylase 1
EC 4.1.1.15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2215-2232Informations de copyright
© 2019 Wiley Periodicals, Inc.