MALDI-TOF MS based procedure to detect KPC-2 directly from positive blood culture bottles and colonies.
Bacterial Proteins
/ metabolism
Bacterial Typing Techniques
/ methods
Escherichia coli
/ chemistry
Escherichia coli Infections
/ microbiology
Humans
Klebsiella Infections
/ microbiology
Klebsiella pneumoniae
/ chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/ methods
beta-Lactamases
/ metabolism
Antimicrobial resistance detection
Blood culture
KPC-2
MALDI-TOF MS
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
14
11
2018
revised:
25
02
2019
accepted:
28
02
2019
pubmed:
9
3
2019
medline:
12
6
2020
entrez:
9
3
2019
Statut:
ppublish
Résumé
In this study, we identified specific carbapenemase-producing isolates applying an easy and rapid protocol for the detection of mature KPC-2 β-lactamase by MALDI-TOF MS from colony and positive blood culture bottles. In addition, we evaluated the correlation of the ~11,109 Da signal as a biomarker associated with KPC-2 production. A collection of 126 well-characterized clinical isolates were evaluated (including 60 KPC-2-producing strains). Presence of KPC-2 was assessed by MALDI-TOF MS on protein extracts. Samples were prepared using the double layer sinapinic acid technique. In order to identify mature KPC-2, raw spectra were analyzed focusing on the range between m/z 25,000-30,000 Da. A single distinctive peak, at approximately m/z 28,544 Da was found in all clinical and control KPC-2-producing strains, and consistently absent in the control groups (ESBL producers and susceptible strains). This peak was detected in all species independently of where the gene bla
Identifiants
pubmed: 30849422
pii: S0167-7012(18)30920-5
doi: 10.1016/j.mimet.2019.02.020
pii:
doi:
Substances chimiques
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
120-127Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.