Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone.
E. coli
H2A-H2B, Ran
Importin-9
histone
histone chaperone
karyopherin
molecular biophysics
nucleosome
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 03 2019
11 03 2019
Historique:
received:
14
11
2018
accepted:
09
03
2019
pubmed:
12
3
2019
medline:
20
3
2020
entrez:
12
3
2019
Statut:
epublish
Résumé
We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome.
Identifiants
pubmed: 30855230
doi: 10.7554/eLife.43630
pii: 43630
pmc: PMC6453568
doi:
pii:
Substances chimiques
Histones
0
IPO9 protein, human
0
Karyopherins
0
Molecular Chaperones
0
Mutant Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01GM112108
Pays : United States
Organisme : NIH HHS
ID : S10 OD021512
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41GM109824
Pays : United States
Organisme : NIH HHS
ID : S10 OD025018
Pays : United States
Organisme : NIGMS NIH HHS
ID : U01GM98256-01
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM112108
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM083960
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM069909
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM069909
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM109824
Pays : United States
Organisme : NIH HHS
ID : S10 OD021596
Pays : United States
Informations de copyright
© 2019, Padavannil et al.
Déclaration de conflit d'intérêts
AP, PS, SK, TC, JJ, CB, DT, AS, SD, YC No competing interests declared
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