Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.
Catechol O-Methyltransferase
/ metabolism
Catechol O-Methyltransferase Inhibitors
/ pharmacology
Catechols
/ pharmacology
Methylation
Methyltransferases
/ metabolism
Nitriles
/ pharmacology
S-Adenosylmethionine
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Tolcapone
/ pharmacology
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
21
06
2018
accepted:
15
02
2019
entrez:
12
3
2019
pubmed:
12
3
2019
medline:
26
11
2019
Statut:
epublish
Résumé
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Identifiants
pubmed: 30856169
doi: 10.1371/journal.pbio.2007050
pii: pbio.2007050
pmc: PMC6411097
doi:
Substances chimiques
Catechol O-Methyltransferase Inhibitors
0
Catechols
0
Nitriles
0
Saccharomyces cerevisiae Proteins
0
entacapone
4975G9NM6T
S-Adenosylmethionine
7LP2MPO46S
Tolcapone
CIF6334OLY
Methyltransferases
EC 2.1.1.-
Catechol O-Methyltransferase
EC 2.1.1.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2007050Déclaration de conflit d'intérêts
I have read the journal's policy, and the authors of this manuscript have the following competing interests. Luo H. is an inventor of the patent applications WO2017202897 and WO2018108966. Luo H. and Hansen A. S. L. are inventors of the patent application WO2018037098.
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