Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour.
Actomyosin
/ antagonists & inhibitors
Animals
Animals, Newborn
Cell Movement
/ drug effects
Cytochalasin D
/ pharmacology
Epithelial Cells
/ drug effects
Heterocyclic Compounds, 4 or More Rings
/ pharmacology
Intravital Microscopy
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Models, Animal
Organogenesis
/ drug effects
Pulmonary Alveoli
/ drug effects
Time-Lapse Imaging
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 03 2019
12 03 2019
Historique:
received:
14
06
2018
accepted:
20
02
2019
entrez:
14
3
2019
pubmed:
14
3
2019
medline:
6
4
2019
Statut:
epublish
Résumé
Damage to alveoli, the gas-exchanging region of the lungs, is a component of many chronic and acute lung diseases. In addition, insufficient generation of alveoli results in bronchopulmonary dysplasia, a disease of prematurity. Therefore visualising the process of alveolar development (alveologenesis) is critical for our understanding of lung homeostasis and for the development of treatments to repair and regenerate lung tissue. Here we show live alveologenesis, using long-term, time-lapse imaging of precision-cut lung slices. We reveal that during this process, epithelial cells are highly mobile and we identify specific cell behaviours that contribute to alveologenesis: cell clustering, hollowing and cell extension. Using the cytoskeleton inhibitors blebbistatin and cytochalasin D, we show that cell migration is a key driver of alveologenesis. This study reveals important novel information about lung biology and provides a new system in which to manipulate alveologenesis genetically and pharmacologically.
Identifiants
pubmed: 30862802
doi: 10.1038/s41467-019-09067-3
pii: 10.1038/s41467-019-09067-3
pmc: PMC6414680
doi:
Substances chimiques
Heterocyclic Compounds, 4 or More Rings
0
blebbistatin
20WC4J7CQ6
Cytochalasin D
22144-77-0
Actomyosin
9013-26-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
1178Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
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