Glatiramer Acetate modulates ion channels expression and calcium homeostasis in B cell of patients with relapsing-remitting multiple sclerosis.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
12 03 2019
Historique:
received: 23 04 2018
accepted: 27 11 2018
entrez: 14 3 2019
pubmed: 14 3 2019
medline: 24 9 2020
Statut: epublish

Résumé

To investigate the effects of Glatiramer Acetate (GA) on B cells by an integrated computational and experimental approach. GA is an immunomodulatory drug approved for the treatment of multiple sclerosis (MS). GA effect on B cells is yet to be fully elucidated. We compared transcriptional profiles of B cells from treatment-naïve relapsing remitting MS patients, treated or not with GA for 6 hours in vitro, and of B cells before and after six months of GA administration in vivo. Microarrays were analyzed with two different computational approaches, one for functional analysis of pathways (Gene Set Enrichment Analysis) and one for the identification of new drug targets (Mode-of-action by Network Analysis). GA modulates the expression of genes involved in immune response and apoptosis. A differential expression of genes encoding ion channels, mostly regulating Ca

Identifiants

pubmed: 30862866
doi: 10.1038/s41598-018-38152-8
pii: 10.1038/s41598-018-38152-8
pmc: PMC6414512
doi:

Substances chimiques

Ion Channels 0
Glatiramer Acetate 5M691HL4BO
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4208

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Auteurs

C Criscuolo (C)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, "Federico II" University of Naples, 80131, Naples, Italy. sky569@hotmail.com.

A Cianflone (A)

IRCCS SDN, Napoli, Italy.

R Lanzillo (R)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, "Federico II" University of Naples, 80131, Naples, Italy.

D Carrella (D)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, (NA), 80078, Italy.

A Carissimo (A)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, (NA), 80078, Italy.
Institute for Applied Mathematics 'Mauro Picone', National Research Council, Naples, Italy.

F Napolitano (F)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, (NA), 80078, Italy.

R de Cegli (R)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, (NA), 80078, Italy.

P de Candia (P)

IRCCS MultiMedica, 20138, Milan, Italy.

C La Rocca (C)

Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy.

T Petrozziello (T)

Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, "Federico II" University of Naples, Naples, Italy.

G Matarese (G)

Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy.
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, 80131, Napoli, Italy.

F Boscia (F)

Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, "Federico II" University of Naples, Naples, Italy.

A Secondo (A)

Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, "Federico II" University of Naples, Naples, Italy.

D Di Bernardo (D)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, (NA), 80078, Italy.
Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, 80125, Naples, Italy.

V Brescia Morra (V)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, "Federico II" University of Naples, 80131, Naples, Italy.

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Classifications MeSH