Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila.
Animals
Cell Line
Chromatin
/ metabolism
Chromatin Assembly and Disassembly
/ genetics
Chromosomes, Insect
/ metabolism
Down-Regulation
Drosophila melanogaster
Gene Expression Profiling
Genes, Insect
/ genetics
Histones
/ metabolism
In Situ Hybridization, Fluorescence
Models, Animal
Nuclear Lamina
/ metabolism
Up-Regulation
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 03 2019
12 03 2019
Historique:
received:
16
02
2018
accepted:
21
02
2019
entrez:
14
3
2019
pubmed:
14
3
2019
medline:
6
4
2019
Statut:
epublish
Résumé
How the nuclear lamina (NL) impacts on global chromatin architecture is poorly understood. Here, we show that NL disruption in Drosophila S2 cells leads to chromatin compaction and repositioning from the nuclear envelope. This increases the chromatin density in a fraction of topologically-associating domains (TADs) enriched in active chromatin and enhances interactions between active and inactive chromatin. Importantly, upon NL disruption the NL-associated TADs become more acetylated at histone H3 and less compact, while background transcription is derepressed. Two-colour FISH confirms that a TAD becomes less compact following its release from the NL. Finally, polymer simulations show that chromatin binding to the NL can per se compact attached TADs. Collectively, our findings demonstrate a dual function of the NL in shaping the 3D genome. Attachment of TADs to the NL makes them more condensed but decreases the overall chromatin density in the nucleus by stretching interphase chromosomes.
Identifiants
pubmed: 30862957
doi: 10.1038/s41467-019-09185-y
pii: 10.1038/s41467-019-09185-y
pmc: PMC6414625
doi:
Substances chimiques
Chromatin
0
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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