124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.
DNA nanotechnology
DNA-PAINT
Super-resolution microscopy
barcoding
multiplexing
Journal
Nano letters
ISSN: 1530-6992
Titre abrégé: Nano Lett
Pays: United States
ID NLM: 101088070
Informations de publication
Date de publication:
10 04 2019
10 04 2019
Historique:
pubmed:
14
3
2019
medline:
2
8
2019
entrez:
14
3
2019
Statut:
ppublish
Résumé
Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.
Identifiants
pubmed: 30864449
doi: 10.1021/acs.nanolett.9b00508
pmc: PMC6463241
doi:
Substances chimiques
Oligonucleotides
0
Proteins
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Pagination
2641-2646Subventions
Organisme : NIBIB NIH HHS
ID : R01 EB018659
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH106011
Pays : United States
Organisme : NIH HHS
ID : 1-U01-MH106011
Pays : United States
Organisme : NIH HHS
ID : R01EB018659
Pays : United States
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