A Novel Fluorescent and Bioluminescent Bireporter Influenza A Virus To Evaluate Viral Infections.
Animals
Antiviral Agents
/ pharmacology
Dogs
Female
Genes, Reporter
/ drug effects
Genetic Engineering
/ methods
Genome, Viral
/ drug effects
HEK293 Cells
Host Microbial Interactions
/ physiology
Humans
Influenza A virus
/ metabolism
Influenza, Human
/ genetics
Luminescent Measurements
/ methods
Luminescent Proteins
/ genetics
Madin Darby Canine Kidney Cells
Mice
Mice, Inbred BALB C
Optical Imaging
/ methods
Orthomyxoviridae Infections
/ virology
Virus Replication
/ drug effects
Nano luciferase
Venus fluorescence
bioluminescence
fluorescence
in vivo expression technology
influenza
reporter genes
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
15 05 2019
15 05 2019
Historique:
received:
08
01
2019
accepted:
04
03
2019
pubmed:
15
3
2019
medline:
3
3
2020
entrez:
15
3
2019
Statut:
epublish
Résumé
Studying influenza A virus (IAV) requires the use of secondary approaches to detect the presence of virus in infected cells. To overcome this problem, we and others have generated recombinant IAV expressing fluorescent or luciferase reporter genes. These foreign reporter genes can be used as valid surrogates to track the presence of virus. However, the limited capacity for incorporating foreign sequences in the viral genome forced researchers to select a fluorescent or a luciferase reporter gene, depending on the type of study. To circumvent this limitation, we engineered a novel recombinant replication-competent bireporter IAV (BIRFLU) expressing both fluorescent and luciferase reporter genes. In cultured cells, BIRFLU displayed growth kinetics comparable to those of wild-type (WT) virus and was used to screen neutralizing antibodies or compounds with antiviral activity. The expression of two reporter genes allows monitoring of viral inhibition by fluorescence or bioluminescence, overcoming the limitations associated with the use of one reporter gene as a readout.
Identifiants
pubmed: 30867298
pii: JVI.00032-19
doi: 10.1128/JVI.00032-19
pmc: PMC6498038
pii:
doi:
Substances chimiques
Antiviral Agents
0
Luminescent Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI141607
Pays : United States
Informations de copyright
Copyright © 2019 American Society for Microbiology.
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