Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase.
Animals
Blotting, Western
/ methods
Chagas Disease
/ metabolism
Flow Cytometry
/ methods
Fluorescent Antibody Technique
/ methods
Glycoproteins
/ metabolism
Host-Parasite Interactions
Humans
Metabolic Networks and Pathways
N-Acetylneuraminic Acid
/ metabolism
Neuraminidase
/ metabolism
Protozoan Proteins
/ metabolism
Staining and Labeling
/ methods
Trypanosoma cruzi
/ enzymology
Mucins
Sialic acid
Sialylglycoconjugates
Trypanosoma cruzi
trans-Sialidase
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
15
3
2019
pubmed:
15
3
2019
medline:
25
7
2019
Statut:
ppublish
Résumé
Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface glycoconjugates from host target cells) by means of a unique trans-sialidase (TS) enzyme. TS-induced changes in the glycoprotein sialylation profile of both parasite and host cells are crucial for the establishment of a persistent T. cruzi infection and for the development of Chagas disease-associated pathogenesis. In this chapter, we describe a novel metabolic labeling method developed in our labs that enables straightforward identification and molecular characterization of SA acceptors of the TS-catalyzed reaction.
Identifiants
pubmed: 30868524
doi: 10.1007/978-1-4939-9148-8_10
pmc: PMC6749821
mid: NIHMS1047420
doi:
Substances chimiques
Glycoproteins
0
Protozoan Proteins
0
trans-sialidase
EC 3.2.1.-
Neuraminidase
EC 3.2.1.18
N-Acetylneuraminic Acid
GZP2782OP0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
135-146Subventions
Organisme : NIAID NIH HHS
ID : R01 AI104531
Pays : United States
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