Differential gene expression for investigation of the effect of germinants and heat activation to induce germination in Bacillus cereus spores.
Bacillus spores
Ger-A, ABC transporters
Heat activation
L-alanine
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
11
08
2018
revised:
10
12
2018
accepted:
22
12
2018
entrez:
20
3
2019
pubmed:
20
3
2019
medline:
27
5
2020
Statut:
ppublish
Résumé
Differential gene expression was used to explore the mechanisms underpinning the differences in the impact of heat activation (70 °C for 30 min) on the germination of Bacillus cereus spores in the presence and absence of a germinant (L-alanine). The number of germinated cells, after heat activation plus L-alanine (3.5 ± 0.02 log CFU/ml) in the spore only initial population was found to be higher than that in only heat activated spores (2.01 ± 0.02 log CFU/ml). The concentration of DPA released by heat activated spores in the presence of L-alanine was 68.3 ± 0.1 and 112.1 ± 0.02 μg/ml after 30 and 60 min, compared to 96.5 and 166.2 ± 0.01 μg/ml after 30 and 90 min, respectively released by spores subjected only to heat activation. Gene (BC0784) encoding for the spore germination protein, gerA operon was up-regulated with a log
Identifiants
pubmed: 30884678
pii: S0963-9969(18)30988-8
doi: 10.1016/j.foodres.2018.12.041
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Membrane Proteins
0
Alanine
OF5P57N2ZX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
462-468Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.