Mesenchymal actomyosin contractility is required for androgen-driven urethral masculinization in mice.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
30
08
2018
accepted:
01
02
2019
entrez:
20
3
2019
pubmed:
20
3
2019
medline:
20
3
2019
Statut:
epublish
Résumé
The morphogenesis of mammalian embryonic external genitalia (eExG) shows dynamic differences between males and females. In genotypic males, eExG are masculinized in response to androgen signaling. Disruption of this process can give rise to multiple male reproductive organ defects. Currently, mechanisms of androgen-driven sexually dimorphic organogenesis are still unclear. We show here that mesenchymal-derived actomyosin contractility, by MYH10, is essential for the masculinization of mouse eExG. MYH10 is expressed prominently in the bilateral mesenchyme of male eExG. Androgen induces MYH10 protein expression and actomyosin contractility in the bilateral mesenchyme. Inhibition of actomyosin contractility through blebbistatin treatment and mesenchymal genetic deletion induced defective urethral masculinization with reduced mesenchymal condensation. We also suggest that actomyosin contractility regulates androgen-dependent mesenchymal directional cell migration to form the condensation in the bilateral mesenchyme leading to changes in urethral plate shape to accomplish urethral masculinization. Thus, mesenchymal-derived actomyosin contractility is indispensable for androgen-driven urethral masculinization.
Identifiants
pubmed: 30886905
doi: 10.1038/s42003-019-0336-3
pii: 336
pmc: PMC6408527
doi:
Substances chimiques
Androgens
0
Biomarkers
0
Actomyosin
9013-26-7
Nonmuscle Myosin Type IIB
EC 3.6.1.-
nonmuscle myosin type IIB heavy chain
EC 3.6.1.-
Myosin Heavy Chains
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
95Déclaration de conflit d'intérêts
The authors declare no competing interests.
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