Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte.
Apoptosis
Cumulus expansion
FGF2
IVM
Meiosis
Oocyte-secreted factors
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
17
11
2018
accepted:
07
03
2019
pubmed:
20
3
2019
medline:
18
12
2019
entrez:
20
3
2019
Statut:
ppublish
Résumé
We first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM. In vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA. FGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6. This is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation.
Identifiants
pubmed: 30887159
doi: 10.1007/s10815-019-01436-7
pii: 10.1007/s10815-019-01436-7
pmc: PMC6541720
doi:
Substances chimiques
Fibroblast Growth Factor 2
103107-01-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
905-913Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 12/06417-8
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 312521/2014-6
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