rSJYB1 inhibits collagen type I protein expression in hepatic stellate cells via down-regulating activity of collagen α1 (I) promoter.
Animals
Cell Line
Collagen Type I
/ genetics
Down-Regulation
/ drug effects
Gene Expression Regulation
/ drug effects
Helminth Proteins
/ genetics
Hepatic Stellate Cells
/ drug effects
Humans
Liver Cirrhosis
/ genetics
Promoter Regions, Genetic
/ genetics
Rabbits
Recombinant Proteins
/ metabolism
Schistosoma japonicum
/ genetics
Y-Box-Binding Protein 1
/ genetics
SJYB1
collagen type I
hepatic stellate cell
liver fibrosis
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
21
12
2018
revised:
28
02
2019
accepted:
01
03
2019
pubmed:
22
3
2019
medline:
21
7
2020
entrez:
22
3
2019
Statut:
ppublish
Résumé
YB1 is a negative regulator in liver fibrosis. We wondered whether SJYB1, a homologous protein of YB1 from Schistosoma japonicum, has an effect on liver fibrosis in vitro. Recombinant SJYB1 (rSJYB1) protein was expressed in a bacterial system and purified by Ni-NTA His·Bind Resin. A human hepatic stellate cell line, the LX-2 cell line, was cultured and treated with rSJYB1. The role of rSJYB1 on LX-2 cells was then analysed by Western blot and luciferase assay. We succeeded in expressing and purifying SJYB1 in a bacterial system and the purified rSJYB1 could be recognized by S japonicum-infected rabbit sera. Western bolt analysis showed that rSJYB1 inhibited the expression of collagen type I, but had little effect on α-smooth muscle actin (α-SMA). Further analysis revealed that rSJYB1 inhibited the activity of collagen α1 (I) (COL1A1) promoter and functioned at -1592/-1176 region of COL1A1 promoter. Our data demonstrate that rSJYB1-mediated anti-fibrotic activity involves inhibiting the activity of COL1A1 promoter and subsequently suppressing the expression of collagen type I in hepatic stellate cells.
Identifiants
pubmed: 30895719
doi: 10.1111/jcmm.14271
pmc: PMC6484296
doi:
Substances chimiques
Collagen Type I
0
Helminth Proteins
0
Recombinant Proteins
0
Y-Box-Binding Protein 1
0
Banques de données
GENBANK
['NC000017.11']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3676-3682Informations de copyright
© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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