Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription.
Animals
Base Sequence
Cell Cycle Proteins
/ metabolism
Embryo, Mammalian
/ cytology
Genes, Tumor Suppressor
Hypothalamus
/ metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
NIH 3T3 Cells
Orexins
/ genetics
Promoter Regions, Genetic
/ genetics
Protein Binding
Transcription Factors
/ metabolism
Transcription, Genetic
Journal
International journal of molecular medicine
ISSN: 1791-244X
Titre abrégé: Int J Mol Med
Pays: Greece
ID NLM: 9810955
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
15
12
2018
accepted:
19
03
2019
pubmed:
22
3
2019
medline:
31
7
2019
entrez:
22
3
2019
Statut:
ppublish
Résumé
The hypocretin/orexin neuropeptide system coordinates the regulation of various physiological processes. Our previous study reported that a reduction in the expression of pleomorphic adenoma gene‑like 1 (Plagl1), which encodes a C2H2 zinc‑finger transcription factor, occurs in hypocretin neuron‑ablated transgenic mice, suggesting that PLAGL1 is co‑expressed in hypocretin neurons and regulates hypocretin transcription. The present study examined whether canonical prepro‑hypocretin transcription is functionally modulated by PLAGL1. Double immunostaining indicated that the majority of hypocretin neurons were positive for PLAGL1 immunoreactivity in the nucleus. Notably, PLAGL1 immunoreactivity in hypocretin neurons was altered in response to several conditions affecting hypocretin function. An uneven localization of PLAGL1 was detected in the nuclei of hypocretin neurons following sleep deprivation. Chromatin immunoprecipitation revealed that endogenous PLAGL1 may bind to a putative PLAGL1‑binding site in the proximal region of the hypocretin gene, in the murine hypothalamus. In addition, electroporation of the PLAGL1 expression vector into the fetal hypothalamus promoted hypothalamic hypocretin transcription. These results suggested that PLAGL1 may regulate hypothalamic hypocretin transcription.
Identifiants
pubmed: 30896835
doi: 10.3892/ijmm.2019.4143
pmc: PMC6445593
doi:
Substances chimiques
Cell Cycle Proteins
0
Orexins
0
Plagl1 protein, mouse
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Pagination
2164-2176Références
J Comp Neurol. 2017 Dec 15;525(18):3809-3820
pubmed: 28608460
Dev Cell. 2006 Nov;11(5):711-22
pubmed: 17084362
Nature. 2007 Jan 11;445(7124):168-76
pubmed: 17151600
Neuropeptides. 2007 Oct;41(5):329-37
pubmed: 17590434
Biochem Biophys Res Commun. 2010 Dec 10;403(2):178-83
pubmed: 21056546
J Neurochem. 2002 Sep;82(6):1561-5
pubmed: 12354306
Nat Rev Neurosci. 2007 Mar;8(3):171-81
pubmed: 17299454
J Neurosci. 2005 Jul 13;25(28):6716-20
pubmed: 16014733
Neuroreport. 2001 Apr 17;12(5):993-7
pubmed: 11303775
Neuroscience. 2006;137(2):593-605
pubmed: 16257491
Neuron. 2018 Feb 7;97(3):520-537.e6
pubmed: 29395907
Circ Res. 2010 Apr 2;106(6):1083-91
pubmed: 20167925
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89
pubmed: 6828386
J Comp Neurol. 1998 Dec 28;402(4):460-74
pubmed: 9862321
Epigenetics. 2017 Sep;12(9):764-778
pubmed: 28762874
Nature. 2009 Dec 3;462(7273):646-50
pubmed: 19956259
Mol Med Rep. 2017 May;15(5):3215-3221
pubmed: 28339047
Vitam Horm. 2012;89:75-90
pubmed: 22640609
Brain Res. 2004 Jan 2;995(1):1-6
pubmed: 14644464
Mol Cell Biol. 2003 Feb;23(3):988-1003
pubmed: 12529403
PLoS One. 2009;4(1):e4254
pubmed: 19158946
J Comp Neurol. 1998 Dec 28;402(4):442-59
pubmed: 9862320
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4459-64
pubmed: 19246384
PLoS One. 2014 Jan 23;9(1):e87478
pubmed: 24466352
J Biol Chem. 2013 Jun 14;288(24):17099-110
pubmed: 23625921
J Biol Chem. 1999 Jun 18;274(25):17771-6
pubmed: 10364220
J Cell Biol. 1999 Sep 20;146(6):1211-26
pubmed: 10491386
Eur J Neurosci. 2005 Jul;22(2):531-4
pubmed: 16045506
Eur J Neurosci. 2009 Jul;30(1):57-64
pubmed: 19508695
Am J Physiol Endocrinol Metab. 2010 Mar;298(3):E726-34
pubmed: 20051529
Eur J Neurosci. 2005 May;21(10):2807-16
pubmed: 15926928
Prog Brain Res. 2012;198:25-46
pubmed: 22813968
Am J Physiol Regul Integr Comp Physiol. 2002 Nov;283(5):R1079-86
pubmed: 12376401
Neuron. 2006 Jun 1;50(5):711-22
pubmed: 16731510
Genes Dev. 2013 Mar 1;27(5):565-78
pubmed: 23431030
Neuron. 2003 Jun 5;38(5):701-13
pubmed: 12797956
Endocr J. 2012;59(5):365-74
pubmed: 22293586
Hum Mol Genet. 2000 Feb 12;9(3):453-60
pubmed: 10655556
EMBO J. 1997 May 15;16(10):2814-25
pubmed: 9184226
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nat Neurosci. 2010 Jun;13(6):767-75
pubmed: 20436479
Mol Med Rep. 2018 Feb;17(2):2263-2270
pubmed: 29207107
Cytogenet Genome Res. 2006;114(2):96-125
pubmed: 16825762
J Biol Chem. 1998 Sep 4;273(36):23026-32
pubmed: 9722527
Exp Neurol. 2005 Apr;192(2):251-64
pubmed: 15755543
Nat Rev Neurosci. 2014 Nov;15(11):719-31
pubmed: 25301357
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8835-40
pubmed: 9671765
Eur J Neurosci. 2001 Oct;14(7):1075-81
pubmed: 11683899
Nucleic Acids Res. 2017 Oct 13;45(18):10466-10480
pubmed: 28985358
Neuroscience. 2011 Jun 2;183:134-43
pubmed: 21435382
J Biol Chem. 2002 May 10;277(19):16985-92
pubmed: 11854267
J Neurosci. 2001 Oct 1;21(19):RC168
pubmed: 11567079