Droplet Digital PCR Detection of the Erythropoietin Transgene from Horse Plasma and Urine for Gene-Doping Control.


Journal

Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097

Informations de publication

Date de publication:
21 03 2019
Historique:
received: 20 02 2019
revised: 16 03 2019
accepted: 16 03 2019
entrez: 24 3 2019
pubmed: 25 3 2019
medline: 25 3 2019
Statut: epublish

Résumé

Indiscriminate genetic manipulation to improve athletic ability is a major threat to human sports and the horseracing industry, in which methods involving gene-doping, such as transgenesis, should be prohibited to ensure fairness. Therefore, development of methods to detect indiscriminate genetic manipulation are urgently needed. Here, we developed a highly sensitive method to detect horse erythropoietin (

Identifiants

pubmed: 30901981
pii: genes10030243
doi: 10.3390/genes10030243
pmc: PMC6471249
pii:
doi:

Substances chimiques

Erythropoietin 11096-26-7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

There are no competing interests including patents, products in development, or marketed products to declare in relationship to this work.

Références

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Auteurs

Teruaki Tozaki (T)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. ttozaki@lrc.or.jp.
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan. ttozaki@lrc.or.jp.

Aoi Ohnuma (A)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. a-ohnuma@lrc.or.jp.

Masaki Takasu (M)

Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan. m-kikuchi@lrc.or.jp.

Mio Kikuchi (M)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. h-kakoi@lrc.or.jp.

Hironaga Kakoi (H)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. k-hirota@lrc.or.jp.

Kei-Ichi Hirota (KI)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. s-nagata@lrc.or.jp.

Kanichi Kusano (K)

Racehorse Hospital Ritto Training Center, Japan Racing Association, 1028 Misono, Ritto, Shiga 520-3085, Japan. takasu@gifu-u.ac.jp.

Shun-Ichi Nagata (SI)

Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan. Kanichi_Kusano@jra.go.jp.

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Classifications MeSH