Proteomic Analysis of FNR-Regulated Anaerobiosis in Salmonella Typhimurium.
Anaerobiosis
Animals
Bacterial Proteins
/ genetics
Caenorhabditis elegans
DNA-Binding Proteins
/ genetics
Gene Expression Regulation, Bacterial
Humans
Oxygen
/ metabolism
Propylene Glycols
/ metabolism
Protein Interaction Maps
Proteomics
Salmonella Infections
/ microbiology
Salmonella typhimurium
/ cytology
Transcription Factors
/ genetics
Anaerobiosis
Quantitative proteomics
Salmonella Typhimurium
The FNR regulon
Journal
Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
02
12
2018
accepted:
24
01
2019
revised:
23
01
2019
pubmed:
25
3
2019
medline:
18
12
2019
entrez:
24
3
2019
Statut:
ppublish
Résumé
Bacterial pathogens such as Salmonella enterica serovar Typhimurium (S. Typhimurium) have to cope with fluctuating oxygen levels during infection within host gastrointestinal tracts. The global transcription factor FNR (fumarate nitrate reduction) plays a vital role in the adaptation of enteric bacteria to the low oxygen environment. Nevertheless, a comprehensive profile of the FNR regulon on the proteome level is still lacking in S. Typhimurium. Herein, we quantitatively profiled S. Typhimurium proteome of an fnr-deletion mutant during anaerobiosis in comparison to its parental strain. Notably, we found that FNR represses the expression of virulence genes of Salmonella pathogenicity island 1 (SPI-1) and negatively regulates propanediol utilization by directly binding to the promoter region of the pdu operon. Importantly, we provided evidence that S. Typhimurium lacking fnr exhibited increased antibiotics susceptibility and membrane permeability as well. Furthermore, genetic deletion of fnr leads to decreased bacterial survival in a Caenorhabditis elegans infection model, highlighting an important role of this regulator in mediating host-pathogen interactions.
Identifiants
pubmed: 30903387
doi: 10.1007/s13361-019-02145-2
pii: 10.1007/s13361-019-02145-2
doi:
Substances chimiques
Bacterial Proteins
0
DNA-Binding Proteins
0
Propylene Glycols
0
Transcription Factors
0
fnr protein, S typhimurium
0
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1001-1012Références
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