Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy.
Acyltransferases
Cell Cycle Proteins
/ chemistry
Corneal Dystrophies, Hereditary
/ genetics
Crystallography, X-Ray
DNA-Binding Proteins
/ chemistry
Humans
Intrinsically Disordered Proteins
/ chemistry
Mutation
/ genetics
Nuclear Proteins
/ chemistry
Protein Binding
/ genetics
Protein Interaction Maps
/ genetics
Retinal Degeneration
/ genetics
Signal Transduction
/ genetics
TEA Domain Transcription Factors
Transcription Factors
/ chemistry
TEAD
YAP
Hippo pathway
Sveinsson's chorioretinal atrophy
intrinsically disordered proteins
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
03
01
2019
revised:
19
02
2019
accepted:
21
03
2019
pubmed:
25
3
2019
medline:
16
5
2020
entrez:
24
3
2019
Statut:
ppublish
Résumé
Four TEAD transcription factors (TEAD1-4) mediate the signalling output of the Hippo pathway that controls organ size in humans. TEAD transcriptional activity is regulated via interactions with the YAP, TAZ and VGLL proteins. A mutation in the TEAD1 gene, Tyr421His, has been identified in patients suffering from Sveinsson's chorioretinal atrophy (SCA), an autosomal dominant eye disease. This mutation prevents the YAP/TAZ:TEAD1 interaction. In this study, we measure the affinity of YAP, TAZ and VGLL1 for the four human TEADs and find that they have a similar affinity for all TEADs. We quantitate the effect of the mutation found in SCA patients and show that it destabilizes the YAP/TAZ:TEAD interaction by about 3 kcal·mol
Substances chimiques
Cell Cycle Proteins
0
DNA-Binding Proteins
0
Intrinsically Disordered Proteins
0
Nuclear Proteins
0
TEA Domain Transcription Factors
0
TEAD1 protein, human
0
Transcription Factors
0
VGLL1 protein, human
0
YY1AP1 protein, human
0
Acyltransferases
EC 2.3.-
TAFAZZIN protein, human
EC 2.3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2381-2398Informations de copyright
© 2019 Federation of European Biochemical Societies.