Frequency of Epstein–Barr Virus DNA in Formalin-Fixed Paraffin-Embedded Tissue of Patients with Ductal Breast Carcinoma
Adult
Breast Neoplasms
/ genetics
Carcinoma, Ductal, Breast
/ genetics
Case-Control Studies
DNA, Viral
/ genetics
Epstein-Barr Virus Infections
/ complications
Epstein-Barr Virus Nuclear Antigens
/ genetics
Female
Fibroadenoma
/ genetics
Follow-Up Studies
Formaldehyde
Herpesvirus 4, Human
/ genetics
Humans
Middle Aged
Paraffin Embedding
Prognosis
Epstein Barr virus
breast cancer
ductal carcinoma
nested PCR
Journal
Asian Pacific journal of cancer prevention : APJCP
ISSN: 2476-762X
Titre abrégé: Asian Pac J Cancer Prev
Pays: Thailand
ID NLM: 101130625
Informations de publication
Date de publication:
26 Mar 2019
26 Mar 2019
Historique:
entrez:
27
3
2019
pubmed:
27
3
2019
medline:
20
7
2019
Statut:
epublish
Résumé
Background: Ductal carcinoma is one of the most common breast cancer (BrC) among the women in the world.
Several factors may involve in establishment of breast cancer. The role of viral infections have been investigated in
BrC, Among them the association of Epstein Barr virus have been reported in the patients with breast cancer type
ductal carcinoma. Thus this study was conducted to evaluate the rate of Epstein Barr virus in women with breast cancer
type ductal carcinoma. Material and methods: A total of 72 formalin-fixed paraffin-embedded tissue blocks samples
were collected from 37 (51.38%) women with breast cancer type ductal carcinoma and 35 (48.61%) samples of breast
with fibro adenoma as control group. The DNA was extracted for all the samples. The detection of EBNA 3C EBV
DNA was done by nested PCR. The results of positive were sequenced to confirm PCR product and determine EBV
genotypes. Results: About 10/37 (27.02%) samples of ductal breast carcinoma were showed positive for EBNA 3C
EBV DNA while 4/35 (11.42%) of fibro adenoma were positive for EBNA 3C EBV DNA (p= 0.095). Randomly 7
PCR products were sequenced and the results of sequencing EBNA 3C shows, the detected EBVDNA were type 1
EBV type. Conclusion: This study shows high prevalence of 27.02% EBV DNA type 1 was found in formalin-fixed
paraffin-embedded tissue of Patients with ductal breast carcinoma. The outcomes of this study suggesting that EBV
might have a significant role in breast cancer in Ahvaz city, south west region of Iran. However the expression of EBV
oncoproteins ,EBNA1, LMP1, and LMP2 require to be determined with ductal carcinoma cells. About 72.97% breast
samples showed negative for EBVDNA. The role other viruses including Human cytomegalovirus, papilloma viruses
and Merkel viruses are required to be investigated in further studies.
Identifiants
pubmed: 30909665
doi: 10.31557/APJCP.2019.20.3.687
pmc: PMC6825768
Substances chimiques
DNA, Viral
0
EBNA-3C, epstein-barr virus
0
Epstein-Barr Virus Nuclear Antigens
0
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
687-692Informations de copyright
Creative Commons Attribution License
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