Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.
Alkaline Phosphatase
/ chemistry
Amino Acid Sequence
Animals
Antibodies, Viral
/ biosynthesis
Antigens, Viral
/ administration & dosage
Chickens
Cross-Linking Reagents
/ chemistry
Enzyme-Linked Immunosorbent Assay
/ methods
Epitopes
/ chemistry
Female
Glutaral
/ chemistry
Humans
Immunization
Immunoconjugates
/ chemistry
Immunoglobulins
/ biosynthesis
Influenza A Virus, H1N1 Subtype
/ chemistry
Influenza A Virus, H3N2 Subtype
/ chemistry
Influenza, Human
/ diagnosis
Nasopharynx
/ virology
Peptides
/ administration & dosage
Spectroscopy, Fourier Transform Infrared
ELISA methods
Immunoglobulin Y
alkaline phosphatase
diagnosis
influenza A
Journal
Bioengineered
ISSN: 2165-5987
Titre abrégé: Bioengineered
Pays: United States
ID NLM: 101581063
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
entrez:
28
3
2019
pubmed:
28
3
2019
medline:
21
8
2019
Statut:
ppublish
Résumé
The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method.
Identifiants
pubmed: 30913952
doi: 10.1080/21655979.2019.1586054
pmc: PMC6527078
doi:
Substances chimiques
Antibodies, Viral
0
Antigens, Viral
0
Cross-Linking Reagents
0
Epitopes
0
IgY
0
Immunoconjugates
0
Immunoglobulins
0
Peptides
0
Alkaline Phosphatase
EC 3.1.3.1
Glutaral
T3C89M417N
Types de publication
Journal Article
Langues
eng
Pagination
33-42Références
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